Amino group in Leptothrix sheath skeleton is responsible for direct deposition of Fe(III) minerals onto the sheaths

Tatsuki Kunoh, Syuji Matsumoto, Noriyuki Nagaoka, Shoko Kanashima, Katsuhiko Hino, Tetsuya Uchida, Katsunori Tamura, Hitoshi Kunoh, Jun Takada

Research output: Contribution to journalArticle

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Abstract

Leptothrix species produce microtubular organic-inorganic materials that encase the bacterial cells. The skeleton of an immature sheath, consisting of organic exopolymer fibrils of bacterial origin, is formed first, then the sheath becomes encrusted with inorganic material. Functional carboxyl groups of polysaccharides in these fibrils are considered to attract and bind metal cations, including Fe(III) and Fe(III)-mineral phases onto the fibrils, but the detailed mechanism remains elusive. Here we show that NH2 of the amino-sugar-enriched exopolymer fibrils is involved in interactions with abiotically generated Fe(III) minerals. NH2-specific staining of L. cholodnii OUMS1 detected a terminal NH2 on its sheath skeleton. Masking NH2 with specific reagents abrogated deposition of Fe(III) minerals onto fibrils. Fe(III) minerals were adsorbed on chitosan and NH2-coated polystyrene beads but not on cellulose and beads coated with an acetamide group. X-ray photoelectron spectroscopy at the N1s edge revealed that the terminal NH2 of OUMS1 sheaths, chitosan and NH2-coated beads binds to Fe(III)-mineral phases, indicating interaction between the Fe(III) minerals and terminal NH2. Thus, the terminal NH2 in the exopolymer fibrils seems critical for Fe encrustation of Leptothrix sheaths. These insights should inform artificial synthesis of highly reactive NH2-rich polymers for use as absorbents, catalysts and so on.

Original languageEnglish
Article number6498
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Minerals
Chitosan
Amino Sugars
Polystyrenes
Cellulose
Functional groups
Polysaccharides
Cations
Polymers
X ray photoelectron spectroscopy
Metals
Catalysts

ASJC Scopus subject areas

  • General

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Amino group in Leptothrix sheath skeleton is responsible for direct deposition of Fe(III) minerals onto the sheaths. / Kunoh, Tatsuki; Matsumoto, Syuji; Nagaoka, Noriyuki; Kanashima, Shoko; Hino, Katsuhiko; Uchida, Tetsuya; Tamura, Katsunori; Kunoh, Hitoshi; Takada, Jun.

In: Scientific Reports, Vol. 7, No. 1, 6498, 01.12.2017.

Research output: Contribution to journalArticle

Kunoh, Tatsuki ; Matsumoto, Syuji ; Nagaoka, Noriyuki ; Kanashima, Shoko ; Hino, Katsuhiko ; Uchida, Tetsuya ; Tamura, Katsunori ; Kunoh, Hitoshi ; Takada, Jun. / Amino group in Leptothrix sheath skeleton is responsible for direct deposition of Fe(III) minerals onto the sheaths. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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