Amino acid sensing using aminoacyl-tRNA synthetase

Akimitsu Kugimiya; Miki Morii; Takashi Ohtsuki

doi:10.1016/j.ab.2008.03.037

Amino acid sensing using aminoacyl-tRNA synthetase

Akimitsu Kugimiya, Miki Morii, Takashi Ohtsuki

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

The detection of amino acids using aminoacyl-tRNA synthetases (ARSs) as the molecular recognition element was proposed, and the binding activity and specificity of ARSs were evaluated. Using this rapid and easy method, from 15 to 50 μM tyrosine could be measured specifically. The method suggested in this article could be realized without an amino acid labeling process or a large volume of organic solvents, and the time for measurement was reasonable.

Original language	English
Pages (from-to)	90-92
Number of pages	3
Journal	Analytical Biochemistry
Volume	378
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2008.03.037
Publication status	Published - Jul 1 2008

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Kugimiya, A., Morii, M., & Ohtsuki, T. (2008). Amino acid sensing using aminoacyl-tRNA synthetase. Analytical Biochemistry, 378(1), 90-92. https://doi.org/10.1016/j.ab.2008.03.037

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