TY - JOUR
T1 - Amino acid sensing using aminoacyl-tRNA synthetase
AU - Kugimiya, Akimitsu
AU - Morii, Miki
AU - Ohtsuki, Takashi
PY - 2008/7/1
Y1 - 2008/7/1
N2 - The detection of amino acids using aminoacyl-tRNA synthetases (ARSs) as the molecular recognition element was proposed, and the binding activity and specificity of ARSs were evaluated. Using this rapid and easy method, from 15 to 50 μM tyrosine could be measured specifically. The method suggested in this article could be realized without an amino acid labeling process or a large volume of organic solvents, and the time for measurement was reasonable.
AB - The detection of amino acids using aminoacyl-tRNA synthetases (ARSs) as the molecular recognition element was proposed, and the binding activity and specificity of ARSs were evaluated. Using this rapid and easy method, from 15 to 50 μM tyrosine could be measured specifically. The method suggested in this article could be realized without an amino acid labeling process or a large volume of organic solvents, and the time for measurement was reasonable.
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U2 - 10.1016/j.ab.2008.03.037
DO - 10.1016/j.ab.2008.03.037
M3 - Article
C2 - 18410738
AN - SCOPUS:43849108907
VL - 378
SP - 90
EP - 92
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -