TY - JOUR
T1 - Amine modification of calcium phosphate by low-pressure plasma for bone regeneration
AU - Kodama, Joe
AU - Harumningtyas, Anjar Anggraini
AU - Ito, Tomoko
AU - Michlíček, Miroslav
AU - Sugimoto, Satoshi
AU - Kita, Hidekazu
AU - Chijimatsu, Ryota
AU - Ukon, Yuichiro
AU - Kushioka, Junichi
AU - Okada, Rintaro
AU - Kamatani, Takashi
AU - Hashimoto, Kunihiko
AU - Tateiwa, Daisuke
AU - Tsukazaki, Hiroyuki
AU - Nakagawa, Shinichi
AU - Takenaka, Shota
AU - Makino, Takahiro
AU - Sakai, Yusuke
AU - Nečas, David
AU - Zajíčková, Lenka
AU - Hamaguchi, Satoshi
AU - Kaito, Takashi
N1 - Funding Information:
T. Kaito has received research funding from Aimedic MMT. This company had no role in the study design, decision to publish, or preparation of the manuscript. Joe Kodama, Anjar Anggraini Harumningtyas, Tomoko Ito, Miroslav Michlíček, Satoshi Sugimoto, Hidekazu Kita, Ryota Chijimatsu, Yuichiro Ukon, Junichi Kushioka, Rintaro Okada, Takashi Kamatani, Kunihiko Hashimoto, Daisuke Tateiwa, Hiroyuki Tsukazaki, Shinichi Nakagawa, Shota Takenaka, Takahiro Makino, Yusuke Sakai, David Nečas, Lenka Zajíčková and Satoshi Hamaguchi declare no potential conflict of interest relevant to this article.
Funding Information:
This work was supported by Innovation Bridge Grant offered by Office for Industry-University Co-creation and University Advancement, Co-creation Bureau, Osaka University [Ja19990016]; the Matching Planner Program, the Regional Industry-Academia Value Program, both offered by Japan Science and Technology Agency (JST); Osaka University International Joint Research Promotion Programs (Type B); Japan Society of the Promotion of Science (JSPS) Grants-in-Aid for Scientific Research (S) [15H05736] and (A)[21H04453]; JSPS Core-to-Core Program [JPJSCCA2019002]; Research Grant of The Nakatomi Foundation; one of the authors (A. A. H) was also financially supported by Research and Innovation in Science and Technology Project (RISET-PRO), Ministry of Research, Technology, and Higher Education of Indonesia; three of the authors (M.M., L.Z. and D.N.) acknowledge CzechNanoLab Research Infrastructure supported by MEYS CR (LM2018110) and the Project 18-12774S supported by the Czech Science Foundation.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is restricted to acting as a bone graft extender owing to the lack of osteogenic ability. To explore whether surface modification might enhance artificial bone functionality, in this study we applied low-pressure plasma technology as next-generation surface treatment and processing strategy to chemically (amine) modify the surface of beta-tricalcium phosphate (β-TCP) artificial bone using a CH4/N2/He gas mixture. Plasma-treated β-TCP exhibited significantly enhanced hydrophilicity, facilitating the deep infiltration of cells into interconnected porous β-TCP. Additionally, cell adhesion and osteogenic differentiation on the plasma-treated artificial bone surfaces were also enhanced. Furthermore, in a rat calvarial defect model, the plasma treatment afforded high bone regeneration capacity. Together, these results suggest that amine modification of artificial bone by plasma technology can provide a high osteogenic ability and represents a promising strategy for resolving current clinical limitations regarding the use of artificial bone.
AB - Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is restricted to acting as a bone graft extender owing to the lack of osteogenic ability. To explore whether surface modification might enhance artificial bone functionality, in this study we applied low-pressure plasma technology as next-generation surface treatment and processing strategy to chemically (amine) modify the surface of beta-tricalcium phosphate (β-TCP) artificial bone using a CH4/N2/He gas mixture. Plasma-treated β-TCP exhibited significantly enhanced hydrophilicity, facilitating the deep infiltration of cells into interconnected porous β-TCP. Additionally, cell adhesion and osteogenic differentiation on the plasma-treated artificial bone surfaces were also enhanced. Furthermore, in a rat calvarial defect model, the plasma treatment afforded high bone regeneration capacity. Together, these results suggest that amine modification of artificial bone by plasma technology can provide a high osteogenic ability and represents a promising strategy for resolving current clinical limitations regarding the use of artificial bone.
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U2 - 10.1038/s41598-021-97460-8
DO - 10.1038/s41598-021-97460-8
M3 - Article
C2 - 34504247
AN - SCOPUS:85114675201
SN - 2045-2322
VL - 11
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 17870
ER -