Ameloblast-lineage cells of rat tooth germs proliferate and scatter in response to hepatocyte growth factor in culture

Tatsushi Matsumura, Makoto J. Tabata, Satoshi Wakisaka, Masayoshi Sakuda, Kojiro Kurisu

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Hepatocyte growth factor (HGF) is considered to be one of the mediators of epithelial-mesenchymal interactions during early organogenesis and to be involved in the development of murine molars. In this study, the immunohistochemical localization of HGF and of its receptor, c-Met, revealed that HGF was distributed in the proliferating mesenchymal cells in the dental papillae and that c-Met was continuously expressed in the epithelial cells during the development of rat incisors. These observations confirmed the involvement of HGF in the development of rat incisors, as demonstrated previously in molars. We then used a primary culture of ameloblast-lineage cells, prepared from mandibular incisors of young rats, to examine the direct effects of HGF on the growth and differentiation of ameloblasts. We found that HGF at 2-20 ng/ml induced a marked increase in the number of ameloblast- lineage cells and in the scattering of such cells. Our results suggest that HGF promotes the proliferation and scattering of ameloblast-lineage cells simultaneously.

Original languageEnglish
Pages (from-to)1137-1142
Number of pages6
JournalInternational Journal of Developmental Biology
Volume42
Issue number8
Publication statusPublished - Nov 1 1998
Externally publishedYes

Keywords

  • Ameloblast-lineage cell
  • Cell proliferation
  • Hepatocyte growth factor
  • Scattering
  • Serum-free culture
  • c-Met

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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