Amelioration of cisplatin-induced acute renal injury by renal progenitor-like cells derived from the adult rat kidney

Masaru Kinomura, Shinji Kitamura, Katsuyuki Tanabe, Kunihiro Ichinose, Kumiko Hirokoshi, Yuki Takazawa, Hiroyuki Kitayama, Tatsuyo Nasu, Hitoshi Sugiyama, Yasushi Yamasaki, Takeshi Sugaya, Yohei Maeshima, Hirofumi Makino

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The replacement of a necrotic tubular epithelium with functional tubular epithelial cells is required for recovery from acute renal failure (ARF). A rat renal progenitor-like (rKS56) cell line was recently established derived from the S3 segment of renal proximal tubules. The therapeutic efficacy of rKS56 cells was examined in a rat model of cisplatin-induced ARF. rKS56-lacZ cells expressing β-galactosidase were injected into SD rats either at the subcapsule of the left kidney (rKS-SC) or via the left renal artery (rKS-IA) 2 days after the injection of cisplatin. Bluo-gal(+) rKS56-lacZ cells were observed in the subcapsule in the rKS-SC group on day 5, and were further increased in number on day 9, accompanied by partial distribution in the corticomedullary junction, but not in the rKS-IA group. A portion of Bluo-gal(+) cells coexpressed Ki-67, aquaporin-1, hepatocyte growth factor (HGF), and c-Met. rKS-SC treatment significantly improved the tubular injury scores, ameliorated tubular cell apoptosis, and induced cell proliferation. The renal function also significantly improved in the rKS-SC group on day 5. These results demonstrate that locally implanted rKS56 cells could differentiate into tubular epithelial cells, thereby accelerating the recovery from tubular injury, most likely by producing tubular trophic factors. These results suggest the therapeutic potential of this novel approach for patients with end-stage renal failure.

Original languageEnglish
Pages (from-to)143-158
Number of pages16
JournalCell Transplantation
Volume17
Issue number1-2
Publication statusPublished - 2008

Fingerprint

Acute Kidney Injury
Cisplatin
Rats
Stem Cells
Kidney
Cells
Recovery
Cell proliferation
Cell death
Epithelial Cells
Aquaporin 1
Galactosidases
Proximal Kidney Tubule
Hepatocyte Growth Factor
Wounds and Injuries
Renal Artery
Chronic Kidney Failure
Therapeutics
Epithelium
Cell Proliferation

Keywords

  • Apoptosis
  • Cisplatin
  • Proliferation
  • Renal progenitor cell
  • Tubular epithelium

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Amelioration of cisplatin-induced acute renal injury by renal progenitor-like cells derived from the adult rat kidney. / Kinomura, Masaru; Kitamura, Shinji; Tanabe, Katsuyuki; Ichinose, Kunihiro; Hirokoshi, Kumiko; Takazawa, Yuki; Kitayama, Hiroyuki; Nasu, Tatsuyo; Sugiyama, Hitoshi; Yamasaki, Yasushi; Sugaya, Takeshi; Maeshima, Yohei; Makino, Hirofumi.

In: Cell Transplantation, Vol. 17, No. 1-2, 2008, p. 143-158.

Research output: Contribution to journalArticle

Kinomura, Masaru ; Kitamura, Shinji ; Tanabe, Katsuyuki ; Ichinose, Kunihiro ; Hirokoshi, Kumiko ; Takazawa, Yuki ; Kitayama, Hiroyuki ; Nasu, Tatsuyo ; Sugiyama, Hitoshi ; Yamasaki, Yasushi ; Sugaya, Takeshi ; Maeshima, Yohei ; Makino, Hirofumi. / Amelioration of cisplatin-induced acute renal injury by renal progenitor-like cells derived from the adult rat kidney. In: Cell Transplantation. 2008 ; Vol. 17, No. 1-2. pp. 143-158.
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