TY - JOUR
T1 - Amelioration of brain edema by topical application of glial cell line- derived neurotrophic factor in reperfused rat brain
AU - Abe, K.
AU - Hayashi, T.
AU - Itoyama, Y.
N1 - Funding Information:
This work was partly supported by Grant-in-Aid for Scientific Research (B) 09470151 from the Ministry of Education, Science and Culture of Japan.
PY - 1997/8/1
Y1 - 1997/8/1
N2 - Glial cell line-derived neurotrophic factor (GDNF) was applied topically on the brain surface of reperfused rat brain after 90 min of transient middle cerebral artery occlusion. In contrast to the cases treated with vehicle, a formation of brain edema was greatly reduced at 2 days by the treatment with GDNF. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL) staining was also markedly reduced in the cases with GDNF treatment both at 1 and 2 days of reperfusion. However, amelioration of the induction of immunoreactive 70 kDa heat shock protein was only a minimum by the GDNF treatment. The present results suggest that the treatment with GDNF has a significant effect on ameliorating brain edema formation after transient focal brain ischemia, and the effect is greatly associated with the reduction of TUNEL staining, but minimally with that of stress response of cells.
AB - Glial cell line-derived neurotrophic factor (GDNF) was applied topically on the brain surface of reperfused rat brain after 90 min of transient middle cerebral artery occlusion. In contrast to the cases treated with vehicle, a formation of brain edema was greatly reduced at 2 days by the treatment with GDNF. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL) staining was also markedly reduced in the cases with GDNF treatment both at 1 and 2 days of reperfusion. However, amelioration of the induction of immunoreactive 70 kDa heat shock protein was only a minimum by the GDNF treatment. The present results suggest that the treatment with GDNF has a significant effect on ameliorating brain edema formation after transient focal brain ischemia, and the effect is greatly associated with the reduction of TUNEL staining, but minimally with that of stress response of cells.
KW - 70 kDa Heat shock protein
KW - Brain edema
KW - Glial cell line-derived neurotrophic factor
KW - Ischemia
KW - TUNEL
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U2 - 10.1016/S0304-3940(97)00517-X
DO - 10.1016/S0304-3940(97)00517-X
M3 - Article
C2 - 9280162
AN - SCOPUS:0030816031
VL - 231
SP - 37
EP - 40
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -