Aluminum tolerance acquired during phosphate starvation in cultured tobacco cells

Yoko Yamamoto, Kazumori Masamoto, Sanae Rikiishi, Akiko Hachiya, Yukiko Yamaguchi, Hideaki Matsumoto

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29 Citations (Scopus)

Abstract

Al toxicity in cultured tobacco cells (Nicotiana tabacum L. cv Samsun; nonchlorophyllic cell line SL) has been investigated in nutrient medium. In this system, Al and Fe(II) (ferrous ion) in the medium synergistically result in the accumulation of both Al and Fe, the peroxidation of lipids, and eventually death in cells at the logarithmic phase of growth (+P cells). A lipophilic antioxidant, N,N'-diphenyl-p-phenylenediamine, protected +P cells from the peroxidation of lipids and from cell death, suggesting that a relationship exists between the two. Compared with +P cells, cells that had been starved of Pi (-P cells) were more tolerant to Al, accumulated 30 to 40% less Al and 70 to 90% less Fe, and did not show any evidence of the peroxidation of lipids during Al treatment. These results suggest that -P cells exhibit Al tolerance because their plasma membranes are protected from the peroxidation of lipids caused by the combination of Al and Fe(II). It seems likely that the exclusion of Fe from -P cells might suppress directly Fe-mediated peroxidation of lipids. Furthermore, since -P cells accumulated β-carotene, it is proposed that this carotenoid pigment might function as a radical-trapping antioxidant in the plasma membrane of cells starved of Pi.

Original languageEnglish
Pages (from-to)217-227
Number of pages11
JournalPlant physiology
Volume112
Issue number1
DOIs
Publication statusPublished - Sep 1996

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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    Yamamoto, Y., Masamoto, K., Rikiishi, S., Hachiya, A., Yamaguchi, Y., & Matsumoto, H. (1996). Aluminum tolerance acquired during phosphate starvation in cultured tobacco cells. Plant physiology, 112(1), 217-227. https://doi.org/10.1104/pp.112.1.217