Aluminum targets elongating cells by reducing cell wall extensibility in wheat roots

Jian Feng Ma, Renfang Shen, Sakiko Nagao, Eiichi Tanimoto

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Phytotoxicity of aluminum is characterized by a rapid inhibition of root elongation at micromolar concentrations, however, the mechanisms primarily responsible for this response are not well understood. We investigated the effect of Al on the viscosity and elasticity parameters of root cell wall by a creep-extension analysis in two cultivars of wheat (Triticum aestivum L.) differing in Al resistance. The root elongation and both viscous and elastic extensibility of cell wall of the root apices were hardly affected by the exposure to 10 μM Al in an Al-resistant cultivar, Atlas 66. However, similar exposure rapidly inhibited root elongation in an Al-sensitive cultivar, Scout 66 and this was associated with a time-dependent accumulation of Al in the root tissues with more than 77% residing in the cell wall. Al caused a significant decrease in both the viscous and elastic extensibility of cell wall of the root apices of Scout 66. The "break load" of the root apex of Scout 66 was also decreased by Al. However, neither the viscosity nor elasticity of the cell wall was affected by in vitro Al treatment. Furthermore, pre-treatment of seedlings with Al in conditions where root elongation was slow (i.e. low temperature) did not affect the subsequent elongation of roots in a 0 Al treatment at room temperature. These results suggest that the Al-dependent changes in the cell wall viscosity and elasticity are involved in the inhibition of root growth. Furthermore, for Al to reduce cell wall extensibility it must interact with the cell walls of actively elongating cells.

Original languageEnglish
Pages (from-to)583-589
Number of pages7
JournalPlant and Cell Physiology
Volume45
Issue number5
DOIs
Publication statusPublished - May 2004
Externally publishedYes

Fingerprint

extensibility
Aluminum
Cell Wall
Triticum
aluminum
cell walls
root growth
wheat
Elasticity
elasticity (mechanics)
Viscosity
cells
viscosity
cultivars
Temperature
Atlases
phytotoxicity
Seedlings
ambient temperature
Triticum aestivum

Keywords

  • Aluminum toxicity
  • Cell wall
  • Extensibility
  • Root elongation
  • Triticum aestivum L.

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Aluminum targets elongating cells by reducing cell wall extensibility in wheat roots. / Ma, Jian Feng; Shen, Renfang; Nagao, Sakiko; Tanimoto, Eiichi.

In: Plant and Cell Physiology, Vol. 45, No. 5, 05.2004, p. 583-589.

Research output: Contribution to journalArticle

Ma, Jian Feng ; Shen, Renfang ; Nagao, Sakiko ; Tanimoto, Eiichi. / Aluminum targets elongating cells by reducing cell wall extensibility in wheat roots. In: Plant and Cell Physiology. 2004 ; Vol. 45, No. 5. pp. 583-589.
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