Aluminum-induced changes in cell-wall glycoproteins in the root tips of al-tolerant and al-sensitive wheat lines

S. S. Kenzhebaeva, Y. Yamamoto, H. Matsumoto

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To investigate wheat (Triticum aestivum L.) responses to AI stress, KCI-and SDS-extracted glycoproteins (covalently bound proteins isolated by cell-wall digestion by cellulysine-pectolase mixture) and extensins (hydroxyproline-containing glycoproteins, HRGPs) were isolated from cell-wall preparations purified from the root apices of Al-sensitive and Al-tolerant near-isogenic lines ES8 and ET8. Under Al stress conditions, two lines differed mostly in their extensins. The untreated plants of two lines were low in covalently bound extensins, although the content of this protein fraction in ES8 was higher than in ET8. When the seedlings were treated with Al, the extensin content increased in both wheat lines and especially in the Al-tolerant ET8 plants. Using two-dimensional electrophoresis, the authors demonstrated the accumulation of polypeptides with mol wts of 22.2 kD (pI 5.5-6.5), 24.5 kD (pI 5.8-6.0), and 33.1 kD (pI 5.25) and polypeptides of 22.2 kD (pI 6.8-7.6) and 40.5 kD (pI 7.6) in the extensin fraction from the cell walls of the Al-sensitive plants. The regulation of cell responses to Al stress may involve extensin expression.

Original languageEnglish
Pages (from-to)441-447
Number of pages7
JournalRussian Journal of Plant Physiology
Volume48
Issue number4
DOIs
Publication statusPublished - Jul 2001

Fingerprint

extensin
root tips
aluminum
glycoproteins
cell walls
wheat
polypeptides
hydroxyproline
isogenic lines
electrophoresis
stress response
Triticum aestivum
digestion
protein content
seedlings

Keywords

  • Al-induced stress
  • Cell wall proteins
  • Root tips
  • Triticum aestivum

ASJC Scopus subject areas

  • Plant Science

Cite this

Aluminum-induced changes in cell-wall glycoproteins in the root tips of al-tolerant and al-sensitive wheat lines. / Kenzhebaeva, S. S.; Yamamoto, Y.; Matsumoto, H.

In: Russian Journal of Plant Physiology, Vol. 48, No. 4, 07.2001, p. 441-447.

Research output: Contribution to journalArticle

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