Abstract
To investigate wheat (Triticum aestivum L.) responses to AI stress, KCI-and SDS-extracted glycoproteins (covalently bound proteins isolated by cell-wall digestion by cellulysine-pectolase mixture) and extensins (hydroxyproline-containing glycoproteins, HRGPs) were isolated from cell-wall preparations purified from the root apices of Al-sensitive and Al-tolerant near-isogenic lines ES8 and ET8. Under Al stress conditions, two lines differed mostly in their extensins. The untreated plants of two lines were low in covalently bound extensins, although the content of this protein fraction in ES8 was higher than in ET8. When the seedlings were treated with Al, the extensin content increased in both wheat lines and especially in the Al-tolerant ET8 plants. Using two-dimensional electrophoresis, the authors demonstrated the accumulation of polypeptides with mol wts of 22.2 kD (pI 5.5-6.5), 24.5 kD (pI 5.8-6.0), and 33.1 kD (pI 5.25) and polypeptides of 22.2 kD (pI 6.8-7.6) and 40.5 kD (pI 7.6) in the extensin fraction from the cell walls of the Al-sensitive plants. The regulation of cell responses to Al stress may involve extensin expression.
| Original language | English |
|---|---|
| Pages (from-to) | 441-447 |
| Number of pages | 7 |
| Journal | Russian Journal of Plant Physiology |
| Volume | 48 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jul 2001 |
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Keywords
- Al-induced stress
- Cell wall proteins
- Root tips
- Triticum aestivum
ASJC Scopus subject areas
- Plant Science
Cite this
Aluminum-induced changes in cell-wall glycoproteins in the root tips of al-tolerant and al-sensitive wheat lines. / Kenzhebaeva, S. S.; Yamamoto, Y.; Matsumoto, H.
In: Russian Journal of Plant Physiology, Vol. 48, No. 4, 07.2001, p. 441-447.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aluminum-induced changes in cell-wall glycoproteins in the root tips of al-tolerant and al-sensitive wheat lines
AU - Kenzhebaeva, S. S.
AU - Yamamoto, Y.
AU - Matsumoto, H.
PY - 2001/7
Y1 - 2001/7
N2 - To investigate wheat (Triticum aestivum L.) responses to AI stress, KCI-and SDS-extracted glycoproteins (covalently bound proteins isolated by cell-wall digestion by cellulysine-pectolase mixture) and extensins (hydroxyproline-containing glycoproteins, HRGPs) were isolated from cell-wall preparations purified from the root apices of Al-sensitive and Al-tolerant near-isogenic lines ES8 and ET8. Under Al stress conditions, two lines differed mostly in their extensins. The untreated plants of two lines were low in covalently bound extensins, although the content of this protein fraction in ES8 was higher than in ET8. When the seedlings were treated with Al, the extensin content increased in both wheat lines and especially in the Al-tolerant ET8 plants. Using two-dimensional electrophoresis, the authors demonstrated the accumulation of polypeptides with mol wts of 22.2 kD (pI 5.5-6.5), 24.5 kD (pI 5.8-6.0), and 33.1 kD (pI 5.25) and polypeptides of 22.2 kD (pI 6.8-7.6) and 40.5 kD (pI 7.6) in the extensin fraction from the cell walls of the Al-sensitive plants. The regulation of cell responses to Al stress may involve extensin expression.
AB - To investigate wheat (Triticum aestivum L.) responses to AI stress, KCI-and SDS-extracted glycoproteins (covalently bound proteins isolated by cell-wall digestion by cellulysine-pectolase mixture) and extensins (hydroxyproline-containing glycoproteins, HRGPs) were isolated from cell-wall preparations purified from the root apices of Al-sensitive and Al-tolerant near-isogenic lines ES8 and ET8. Under Al stress conditions, two lines differed mostly in their extensins. The untreated plants of two lines were low in covalently bound extensins, although the content of this protein fraction in ES8 was higher than in ET8. When the seedlings were treated with Al, the extensin content increased in both wheat lines and especially in the Al-tolerant ET8 plants. Using two-dimensional electrophoresis, the authors demonstrated the accumulation of polypeptides with mol wts of 22.2 kD (pI 5.5-6.5), 24.5 kD (pI 5.8-6.0), and 33.1 kD (pI 5.25) and polypeptides of 22.2 kD (pI 6.8-7.6) and 40.5 kD (pI 7.6) in the extensin fraction from the cell walls of the Al-sensitive plants. The regulation of cell responses to Al stress may involve extensin expression.
KW - Al-induced stress
KW - Cell wall proteins
KW - Root tips
KW - Triticum aestivum
UR - http://www.scopus.com/inward/record.url?scp=0035536146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035536146&partnerID=8YFLogxK
U2 - 10.1023/A:1016786926646
DO - 10.1023/A:1016786926646
M3 - Article
AN - SCOPUS:0035536146
VL - 48
SP - 441
EP - 447
JO - Russian Journal of Plant Physiology
JF - Russian Journal of Plant Physiology
SN - 1021-4437
IS - 4
ER -