Alternative oxidase in resistance to biotic stresses: Nicotiana attenuata AOX contributes to resistance to a pathogen and a piercing-sucking insect but not Manduca sexta Larvae

Lu Zhang, Youngjoo Oh, Hongyu Li, Ian T. Baldwin, Ivan Galis

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The role of the alternative respiratory pathway in the protection of plants against biotic stress was examined in transgenic tobacco (Nicotiana attenuata) plants (irAOX) silenced in the expression of ALTERNATIVE OXIDASE (AOX) gene. Wild-type and irAOX plants were independently challenged with (1) chewing herbivores (Manduca sexta), (2) piercing-sucking insects (Empoasca spp.), and (3) bacterial pathogens (Pseudomonas syringae pv tomato DC3000), showing that all these treatments can strongly elicit accumulation of AOX gene transcripts in wild-type plants. When N. attenuata chemical defenses and resistance were examined, irAOX plants showed wild-type levels of defense-related phytohormones, secondary metabolites, and resistance to M. sexta. In contrast, piercing-sucking leafhoppers (Empoasca spp.) caused more leaf damage and induced significantly higher salicylic acid levels in irAOX compared with wild-type plants in the field and/or glasshouse. Subsequently, irAOX plants accumulated lower levels of defense metabolites, 17-hydroxygeranyllinalool diterpene glycosides, caffeoylputrescine, and nicotine compared with wild-type plants under prolonged attack of Empoasca spp. in the glasshouse. Finally, an accelerated cell death phenotype was observed in irAOX plants infected with P. syringae, which correlated with higher levels of salicylic acid and hydrogen peroxide levels in pathogen-infected irAOX compared with wild-type leaves. Overall, the AOX-associated changes in phytohormone and/or redox levels appear to support the resistance of N. attenuata plants against cell piercing-sucking insects and modulate the progression of cell death in pathogen-infected tissues but are not effective against rapidly feeding specialist herbivore M. sexta.

Original languageEnglish
Pages (from-to)1453-1467
Number of pages15
JournalPlant Physiology
Volume160
Issue number3
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Manduca
Manduca sexta
biotic stress
Tobacco
Larva
Insects
insects
pathogens
larvae
Empoasca
Pseudomonas syringae
Herbivory
Plant Growth Regulators
Salicylic Acid
salicylic acid
plant hormones
cell death
herbivores
Cell Death
greenhouses

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Alternative oxidase in resistance to biotic stresses : Nicotiana attenuata AOX contributes to resistance to a pathogen and a piercing-sucking insect but not Manduca sexta Larvae. / Zhang, Lu; Oh, Youngjoo; Li, Hongyu; Baldwin, Ian T.; Galis, Ivan.

In: Plant Physiology, Vol. 160, No. 3, 11.2012, p. 1453-1467.

Research output: Contribution to journalArticle

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