Alternative methods to evaluate the protective ability of sunscreen against photo-genotoxicity

Megumi Toyoshima, Kanako Hosoda, Miho Hanamura, Keinosuke Okamoto, Hiroshi Kobayashi, Tomoe Negishi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Numerous epidemiological investigations show that sunlight is carcinogenic to humans and that the use of sunscreen may be effective in decreasing the risk of skin cancer. The biological activity of a sunscreen is evaluated by its ability to protect human skin from erythema as represented by a Sun Protection Factor (SPF). We propose that the sunscreen's protective effect against sunlight-induced genotoxicity, including mutation, should also be taken into account. In this study we examined the protective ability of sunscreens against natural sunlight and UV-induced genotoxicity in Drosophila somatic cells. We prepared three kinds of sunscreen samples, each with an SPF value of 20, 40 or 60 and compared their protective activities with commercial sunscreens. When a sunscreen of SPF 20, 40 or 60 was pasted on the plastic cover of a petri dish in which Drosophila larvae were exposed to the sun or UV lamps, genotoxicity decreased as the SPF of the sunscreen increased, relative to levels of genotoxicity observed in samples without sunscreen. However, the protective abilities of sunscreens were unexpectedly not so different from each other. To reveal the relationship between the protective activity of sunscreen and the wavelength of light with which larvae were irradiated through the sunscreen, we measured the transmittance of light through the petri dish cover on which the sunscreen was pasted. Effective protection was demonstrated by removing components of light whose wavelengths were below 315 nm. We suggest, that the measurement of anti-genotoxic activity and the determination of the wavelengths of light transmitted through the sunscreen should be an alternative method for evaluating the effectiveness of a sunscreen.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalJournal of Photochemistry and Photobiology B: Biology
Volume73
Issue number1-2
DOIs
Publication statusPublished - Jan 23 2004

Fingerprint

Sun hoods
Sunscreening Agents
genotoxicity
sun
sunlight
larvae
Drosophila
parabolic reflectors
Sun Protection Factor
Sun
wavelengths
methodology
Sunlight
mutations
activity (biology)
luminaires
Light
transmittance
solar radiation
plastics

Keywords

  • Drosophila melanogaster
  • Genotoxicity
  • Somatic cell mutation
  • Sunlight
  • Sunscreen
  • Transmittance
  • UVB

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Alternative methods to evaluate the protective ability of sunscreen against photo-genotoxicity. / Toyoshima, Megumi; Hosoda, Kanako; Hanamura, Miho; Okamoto, Keinosuke; Kobayashi, Hiroshi; Negishi, Tomoe.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 73, No. 1-2, 23.01.2004, p. 59-66.

Research output: Contribution to journalArticle

Toyoshima, Megumi ; Hosoda, Kanako ; Hanamura, Miho ; Okamoto, Keinosuke ; Kobayashi, Hiroshi ; Negishi, Tomoe. / Alternative methods to evaluate the protective ability of sunscreen against photo-genotoxicity. In: Journal of Photochemistry and Photobiology B: Biology. 2004 ; Vol. 73, No. 1-2. pp. 59-66.
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