Altered response to formalin by L5 spinal nerve ligation in rats

A behavioral and molecular study

Ryuji Kaku, Masataka Yokoyama, Hiroyuki Kobayashi, Yoshikazu Matsuoka, Tetsufumi Sato, Satoshi Mizobuchi, Yoshitaro Itano, Kiyoshi Morita

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

BACKGROUND: The status of neuropathic pain alters the responsiveness to formalin injection in rats. However, the mechanism by which this alteration occurs is unknown. METHODS: We used immunocytochemistry to examine the expression of brain-derived neurotrophic factor (BDNF) and calcitonin gene-related peptide (CGRP) in the spinal cord of rats with L5 spinal nerve ligation (SNL)-induced neuropathy, and investigated the expression of c-Fos in the spinal cord after injection of formalin in the hindpaw of rats with SNL. RESULTS: Four weeks after SNL, the withdrawal threshold was significantly lower in the SNL group than in the sham-operated (sham) group (n = 12 per group, P <0.05). In the SNL group, expression of BDNF in the L4 (P <0.05) and L5 (P <0.01) superficial dorsal horn was significantly decreased compared to that in the sham group. CGRP protein in the L5 but not in the L4, dorsal horn was significantly decreased compared to that in the sham group (P <0.01). After formalin injection, spontaneous pain responses in the SNL group were significantly decreased compared to those in the sham group (P <0.05). Immunolabeling for c-Fos was significantly decreased in the L4 and L5 dorsal horn in the SNL group (P <0.01). CONCLUSION: Our examination of c-Fos distribution indicates that decreased neuronal activity in the spinal cord in response to inflammatory pain may be important for altering the perception of acute pain. Decreased BDNF expression in response to SNL-induced neuropathy may be involved in this alteration.

Original languageEnglish
Pages (from-to)936-943
Number of pages8
JournalAnesthesia and Analgesia
Volume104
Issue number4
DOIs
Publication statusPublished - Apr 2007

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Spinal Nerves
Formaldehyde
Ligation
Brain-Derived Neurotrophic Factor
Spinal Cord
Calcitonin Gene-Related Peptide
Injections
Pain
Acute Pain
Neuralgia
Immunohistochemistry

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Altered response to formalin by L5 spinal nerve ligation in rats : A behavioral and molecular study. / Kaku, Ryuji; Yokoyama, Masataka; Kobayashi, Hiroyuki; Matsuoka, Yoshikazu; Sato, Tetsufumi; Mizobuchi, Satoshi; Itano, Yoshitaro; Morita, Kiyoshi.

In: Anesthesia and Analgesia, Vol. 104, No. 4, 04.2007, p. 936-943.

Research output: Contribution to journalArticle

Kaku, Ryuji ; Yokoyama, Masataka ; Kobayashi, Hiroyuki ; Matsuoka, Yoshikazu ; Sato, Tetsufumi ; Mizobuchi, Satoshi ; Itano, Yoshitaro ; Morita, Kiyoshi. / Altered response to formalin by L5 spinal nerve ligation in rats : A behavioral and molecular study. In: Anesthesia and Analgesia. 2007 ; Vol. 104, No. 4. pp. 936-943.
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