Altered nano/micro-order elasticity of pulmonary artery smooth muscle cells of patients with idiopathic pulmonary arterial hypertension

Kazufumi Nakamura, Juichiro Shimizu, Noriyuki Kataoka, Katsushi Hashimoto, Tetsuya Ikeda, Hideki Fujio, Keiko Ohta-Ogo, Aiko Ogawa, Aya Miura, Satoshi Mohri, Satoshi Nagase, Hiroshi Morita, Kengo Fukushima Kusano, Hiroshi Date, Hiromi Matsubara, Seiichi Mochizuki, Ken Hashimoto, Fumihiko Kajiya, Tohru Ohe

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Idiopathic pulmonary arterial hypertension (IPAH) is a disease characterized by progressively increased resistance of pulmonary arteries. In this study, we evaluated the mechanical property of single pulmonary artery smooth muscles cells (PASMC) from patients with IPAH and tested whether the PASMC showed abnormal response to a vasodilator by use of an atomic force microscope (AFM). Methods: PASMC were isolated and cultured from explanted lungs of 7 patients with IPAH (IPAH-PASMC). Normal vascular specimens from 3 patients with bronchogenic carcinoma were used as normal controls (normal PASMC). The nano/micro-order elasticity of five to ten living PASMC in each sample was measured by parabolic force curves of cantilever deflection/indentation obtained by using an AFM. The elasticity measurements were performed under control conditions and under condition of nitric oxide (NO) treatment (190 and 380 nmol/L). Results: There was no significant difference between nano/micro-order elasticity of normal PASMC and that of IPAH-PASMC under the control conditions. In normal PASMC, NO (190 and 380 nmol/L) significantly reduced (i.e., softened) the nano/micro-order elasticity. However, NO did not reduce elasticity in IPAH-PASMC, indicating higher vasodilator-resistive nano/micro-order rigidity in IPAH-PASMC. Conclusion: Nano/micro-order elasticity change in PASMC in response to vasodilation induced by NO is reduced in patients with IPAH.

Original languageEnglish
Pages (from-to)102-107
Number of pages6
JournalInternational Journal of Cardiology
Volume140
Issue number1
DOIs
Publication statusPublished - Apr 1 2010

Fingerprint

Elasticity
Pulmonary Artery
Smooth Muscle Myocytes
Nitric Oxide
Vasodilator Agents
Familial Primary Pulmonary Hypertension
Bronchogenic Carcinoma
Vasodilation
Blood Vessels

Keywords

  • Atomic force microscope
  • Nitric oxide
  • Pulmonary hypertension
  • Smooth muscle cell
  • Vasodilator

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Altered nano/micro-order elasticity of pulmonary artery smooth muscle cells of patients with idiopathic pulmonary arterial hypertension. / Nakamura, Kazufumi; Shimizu, Juichiro; Kataoka, Noriyuki; Hashimoto, Katsushi; Ikeda, Tetsuya; Fujio, Hideki; Ohta-Ogo, Keiko; Ogawa, Aiko; Miura, Aya; Mohri, Satoshi; Nagase, Satoshi; Morita, Hiroshi; Kusano, Kengo Fukushima; Date, Hiroshi; Matsubara, Hiromi; Mochizuki, Seiichi; Hashimoto, Ken; Kajiya, Fumihiko; Ohe, Tohru.

In: International Journal of Cardiology, Vol. 140, No. 1, 01.04.2010, p. 102-107.

Research output: Contribution to journalArticle

Nakamura, K, Shimizu, J, Kataoka, N, Hashimoto, K, Ikeda, T, Fujio, H, Ohta-Ogo, K, Ogawa, A, Miura, A, Mohri, S, Nagase, S, Morita, H, Kusano, KF, Date, H, Matsubara, H, Mochizuki, S, Hashimoto, K, Kajiya, F & Ohe, T 2010, 'Altered nano/micro-order elasticity of pulmonary artery smooth muscle cells of patients with idiopathic pulmonary arterial hypertension', International Journal of Cardiology, vol. 140, no. 1, pp. 102-107. https://doi.org/10.1016/j.ijcard.2008.11.022
Nakamura, Kazufumi ; Shimizu, Juichiro ; Kataoka, Noriyuki ; Hashimoto, Katsushi ; Ikeda, Tetsuya ; Fujio, Hideki ; Ohta-Ogo, Keiko ; Ogawa, Aiko ; Miura, Aya ; Mohri, Satoshi ; Nagase, Satoshi ; Morita, Hiroshi ; Kusano, Kengo Fukushima ; Date, Hiroshi ; Matsubara, Hiromi ; Mochizuki, Seiichi ; Hashimoto, Ken ; Kajiya, Fumihiko ; Ohe, Tohru. / Altered nano/micro-order elasticity of pulmonary artery smooth muscle cells of patients with idiopathic pulmonary arterial hypertension. In: International Journal of Cardiology. 2010 ; Vol. 140, No. 1. pp. 102-107.
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abstract = "Background: Idiopathic pulmonary arterial hypertension (IPAH) is a disease characterized by progressively increased resistance of pulmonary arteries. In this study, we evaluated the mechanical property of single pulmonary artery smooth muscles cells (PASMC) from patients with IPAH and tested whether the PASMC showed abnormal response to a vasodilator by use of an atomic force microscope (AFM). Methods: PASMC were isolated and cultured from explanted lungs of 7 patients with IPAH (IPAH-PASMC). Normal vascular specimens from 3 patients with bronchogenic carcinoma were used as normal controls (normal PASMC). The nano/micro-order elasticity of five to ten living PASMC in each sample was measured by parabolic force curves of cantilever deflection/indentation obtained by using an AFM. The elasticity measurements were performed under control conditions and under condition of nitric oxide (NO) treatment (190 and 380 nmol/L). Results: There was no significant difference between nano/micro-order elasticity of normal PASMC and that of IPAH-PASMC under the control conditions. In normal PASMC, NO (190 and 380 nmol/L) significantly reduced (i.e., softened) the nano/micro-order elasticity. However, NO did not reduce elasticity in IPAH-PASMC, indicating higher vasodilator-resistive nano/micro-order rigidity in IPAH-PASMC. Conclusion: Nano/micro-order elasticity change in PASMC in response to vasodilation induced by NO is reduced in patients with IPAH.",
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AU - Nakamura, Kazufumi

AU - Shimizu, Juichiro

AU - Kataoka, Noriyuki

AU - Hashimoto, Katsushi

AU - Ikeda, Tetsuya

AU - Fujio, Hideki

AU - Ohta-Ogo, Keiko

AU - Ogawa, Aiko

AU - Miura, Aya

AU - Mohri, Satoshi

AU - Nagase, Satoshi

AU - Morita, Hiroshi

AU - Kusano, Kengo Fukushima

AU - Date, Hiroshi

AU - Matsubara, Hiromi

AU - Mochizuki, Seiichi

AU - Hashimoto, Ken

AU - Kajiya, Fumihiko

AU - Ohe, Tohru

PY - 2010/4/1

Y1 - 2010/4/1

N2 - Background: Idiopathic pulmonary arterial hypertension (IPAH) is a disease characterized by progressively increased resistance of pulmonary arteries. In this study, we evaluated the mechanical property of single pulmonary artery smooth muscles cells (PASMC) from patients with IPAH and tested whether the PASMC showed abnormal response to a vasodilator by use of an atomic force microscope (AFM). Methods: PASMC were isolated and cultured from explanted lungs of 7 patients with IPAH (IPAH-PASMC). Normal vascular specimens from 3 patients with bronchogenic carcinoma were used as normal controls (normal PASMC). The nano/micro-order elasticity of five to ten living PASMC in each sample was measured by parabolic force curves of cantilever deflection/indentation obtained by using an AFM. The elasticity measurements were performed under control conditions and under condition of nitric oxide (NO) treatment (190 and 380 nmol/L). Results: There was no significant difference between nano/micro-order elasticity of normal PASMC and that of IPAH-PASMC under the control conditions. In normal PASMC, NO (190 and 380 nmol/L) significantly reduced (i.e., softened) the nano/micro-order elasticity. However, NO did not reduce elasticity in IPAH-PASMC, indicating higher vasodilator-resistive nano/micro-order rigidity in IPAH-PASMC. Conclusion: Nano/micro-order elasticity change in PASMC in response to vasodilation induced by NO is reduced in patients with IPAH.

AB - Background: Idiopathic pulmonary arterial hypertension (IPAH) is a disease characterized by progressively increased resistance of pulmonary arteries. In this study, we evaluated the mechanical property of single pulmonary artery smooth muscles cells (PASMC) from patients with IPAH and tested whether the PASMC showed abnormal response to a vasodilator by use of an atomic force microscope (AFM). Methods: PASMC were isolated and cultured from explanted lungs of 7 patients with IPAH (IPAH-PASMC). Normal vascular specimens from 3 patients with bronchogenic carcinoma were used as normal controls (normal PASMC). The nano/micro-order elasticity of five to ten living PASMC in each sample was measured by parabolic force curves of cantilever deflection/indentation obtained by using an AFM. The elasticity measurements were performed under control conditions and under condition of nitric oxide (NO) treatment (190 and 380 nmol/L). Results: There was no significant difference between nano/micro-order elasticity of normal PASMC and that of IPAH-PASMC under the control conditions. In normal PASMC, NO (190 and 380 nmol/L) significantly reduced (i.e., softened) the nano/micro-order elasticity. However, NO did not reduce elasticity in IPAH-PASMC, indicating higher vasodilator-resistive nano/micro-order rigidity in IPAH-PASMC. Conclusion: Nano/micro-order elasticity change in PASMC in response to vasodilation induced by NO is reduced in patients with IPAH.

KW - Atomic force microscope

KW - Nitric oxide

KW - Pulmonary hypertension

KW - Smooth muscle cell

KW - Vasodilator

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