Altered electrolyte handling of the choroid plexus in rats with glycerol-induced acute renal failure

Atsuko Ishikawa, Kentaro Kono, Rie Sakae, Tetsuya Aiba, Hiromu Kawasaki, Yuji Kurosaki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The altered electrolyte handling of the choroid plexus was investigated in rats with acute renal failure (ARF) using lithium and rubidium as surrogate markers for sodium and potassium, respectively. Firstly, the transport of these two markers from the plasma to cerebrospinal fluid (CSF) was evaluated after they were concurrently injected into the femoral vein. As a result, their disposition from the plasma to CSF was shown to decrease in ARF rats, but the relationship profile between those two markers was not different from that observed in normal rats, indicating that the decreased disposition of lithium and rubidium occurs without affecting the stoichiometric balance. To clarify the mechanisms accounting for the decreased disposition, an inhibition study was then performed. When bumetanide, an inhibitor of the Na+/K +/2Cl- co-transporter, was directly introduced into the cerebroventricle prior to lithium and rubidium being intravenously administered, a marked increase in the markers' disposition was observed. However, such an increased disposition did not occur when bumetanide was injected into the femoral vein. Other inhibitors, such as amiloride for the Na+/H + exchanger and ouabain for Na+/K+-ATPase, did not show any effects on marker disposition regardless of the inhibitor being administered into either the cerebroventricle or femoral vein. These findings suggest that the decreased marker disposition in ARF rats is due to an increased efflux process of the choroid plexus mediated by the Na+/K +/2Cl- co-transporter. That is, electrolyte efflux from the CSF to plasma increases, and thereby the electrolyte influx from the plasma to CSF is counteracted.

Original languageEnglish
Pages (from-to)455-463
Number of pages9
JournalBiopharmaceutics and Drug Disposition
Volume31
Issue number8-9
DOIs
Publication statusPublished - Nov 1 2010

Keywords

  • NKCC1
  • acute renal failure
  • cerebrospinal fluid
  • lithium
  • rubidium

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

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