Altered carbohydrate metabolism in the storage roots of sweetpotato plants overexpressing the SRF1 gene, which encodes a Dof zinc finger transcription factor

Masaru Tanaka, Yasuhiro Takahata, Hiroki Nakayama, Makoto Nakatani, Makoto Tahara

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

In order to characterize the functions of the sweetpotato SRF1 gene, which encodes a Dof zing finger transcriptional factor preferentially expressed in the storage roots, we isolated its full length cDNA and produced transgenic sweetpotato plants with altered SRF1 expression levels. The isolated cDNA of SRF1 encoded a polypeptide of 497 amino acids and was closely related to the cyclic Dof factors of Arabidopsis and the ascorbate oxidase binding protein of pumpkin. SRF1 was most highly expressed in storage roots, although some expression was also observed in other vegetative tissue. Transgenic plants overexpressing SRF1 showed significantly higher storage root dry matter content compared to the original cultivar Kokei No. 14 or control transgenic plants. In these plants, the starch content per fresh weight of the storage roots was also higher than that of the wild-type plants, while the glucose and fructose content drastically decreased. Among the enzymes involved in the sugar metabolism, soluble acid invertase showed a decreased activity in the transgenic plants. Gene expression analysis showed that the expression of Ibβfruct2, which encodes an isoform of vacuolar invertase, was suppressed in the transgenic plants overexpressing the SRF1 gene. These data suggest that SRF1 modulates the carbohydrate metabolism in the storage roots through negative regulation of a vacuolar invertase gene.

Original languageEnglish
Pages (from-to)737-746
Number of pages10
JournalPlanta
Volume230
Issue number4
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Plant Roots
zinc finger motif
Genetically Modified Plants
Zinc Fingers
Carbohydrate Metabolism
carbohydrate metabolism
Transcription Factors
beta-Fructofuranosidase
transcription factors
transgenic plants
beta-fructofuranosidase
Genes
genes
Ascorbate Oxidase
Complementary DNA
ascorbate oxidase
Cucurbita
pumpkins
Fructose
dry matter content

Keywords

  • Dof protein
  • Dry matter content
  • Invertase
  • Ipomoea batatas
  • Starch
  • Transformation

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

Altered carbohydrate metabolism in the storage roots of sweetpotato plants overexpressing the SRF1 gene, which encodes a Dof zinc finger transcription factor. / Tanaka, Masaru; Takahata, Yasuhiro; Nakayama, Hiroki; Nakatani, Makoto; Tahara, Makoto.

In: Planta, Vol. 230, No. 4, 09.2009, p. 737-746.

Research output: Contribution to journalArticle

Tanaka, Masaru ; Takahata, Yasuhiro ; Nakayama, Hiroki ; Nakatani, Makoto ; Tahara, Makoto. / Altered carbohydrate metabolism in the storage roots of sweetpotato plants overexpressing the SRF1 gene, which encodes a Dof zinc finger transcription factor. In: Planta. 2009 ; Vol. 230, No. 4. pp. 737-746.
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