Alterations in photosynthetic pigments and amino acid composition of D1 protein change energy distribution in photosystem II

Makio Yokono, Tatsuya Tomo, Ryo Nagao, Hisashi Ito, Ayumi Tanaka, Seiji Akimoto

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The marine cyanobacterium Prochlorococcus marinus accumulates divinyl chlorophylls instead of monovinyl chlorophylls to harvest light energy. As well as this difference in its chromophore composition, some amino acid residues in its photosystem II D1 protein were different from the conserved amino acid residues in other photosynthetic organisms. We examined PSII complexes isolated from mutants of Synechocystis sp. PCC 6803, in which chromophore and D1 protein were altered (Hisashi Ito and Ayumi Tanaka, 2011) to clarify the effects of chromophores/D1 protein composition on the excitation energy distribution. We prepared the mutants accumulating divinyl chlorophyll (DV mutant). The amino acid residues of V205 and G282 in the D1 protein were substituted with M205 and C282 in the DV mutant to mimic Prochlorococcus D1 protein (DV-V205M/G282C mutant). Isolated PSII complexes were analyzed by time-resolved fluorescence spectroscopy. Energy transfer in CP47 was interrupted in PSII containing divinyl chlorophylls. The V205M/G282C mutation did not recover the energy transfer pathway in CP47, instead, the mutation allowed the excitation energy transfer from CP43 to CP47, which neighbors in the PSII dimer. Mutual orientation of the subcomplexes of PSII might be affected by the substitution. The changes of the energy transfer pathways would reduce energy transfer from antennae to the PSII reaction center, and allow Prochlorococcus to acquire light tolerance.

Original languageEnglish
Pages (from-to)754-759
Number of pages6
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1817
Issue number5
DOIs
Publication statusPublished - May 2012
Externally publishedYes

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Photosystem II Protein Complex
Energy Transfer
Prochlorococcus
Pigments
Energy transfer
Chlorophyll
Amino Acids
Chromophores
Chemical analysis
Excitation energy
Proteins
Synechocystis
Light
Mutation
Fluorescence Spectrometry
Fluorescence spectroscopy
Cyanobacteria
Dimers
Substitution reactions
Antennas

Keywords

  • Cyanobacteria
  • Delayed fluorescence
  • Divinyl chlorophyll
  • Photosystem II

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Alterations in photosynthetic pigments and amino acid composition of D1 protein change energy distribution in photosystem II. / Yokono, Makio; Tomo, Tatsuya; Nagao, Ryo; Ito, Hisashi; Tanaka, Ayumi; Akimoto, Seiji.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1817, No. 5, 05.2012, p. 754-759.

Research output: Contribution to journalArticle

Yokono, Makio ; Tomo, Tatsuya ; Nagao, Ryo ; Ito, Hisashi ; Tanaka, Ayumi ; Akimoto, Seiji. / Alterations in photosynthetic pigments and amino acid composition of D1 protein change energy distribution in photosystem II. In: Biochimica et Biophysica Acta - Bioenergetics. 2012 ; Vol. 1817, No. 5. pp. 754-759.
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