Alterations in glomerular extracellular matrix components in glomerulonephritis

Shuji Ikeda, Hirofumi Makino, Zensuke Ota

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We investigated alterations in the main components of glomerular extracellular matrices, including heparansulfate proteoglycan (HS-PG), laminin, type IV collagen and fibronectin in the renal tissues of 61 patients with various types of glomerulonephritis. Indirect immunofluorescence stainings with polyclonal antibodies of these extracellular matrix components were performed. In minimal change nephrotic syndrome, no remarkable changes were observed. In membranous glomerulonephritis, non-collagenous components, such as HS-PG and laminin altered in distribution, forming spikes in stage II and circles between or around the immune deposits in stage III. These changes were observed more clearly in HS-PG and laminin than in type IV collagen. These results suggested that non-collagenous components played an important role in repairing the GBM. In membranoproliferati ve glomerulonephritis and IgA nephropathy, type IV collagen and fibronectin expanded in the proliferated mesangial area. These findings showed that there was a close relationship between these extracellular matrix and the progression of glomerulosclerosis. In dense deposit disease, double contour of staining of HS-PG, laminin and type IV collagen was observed along the capillary wall. These findings suggested that there were no main extracellular matrix components within the dense materials.

Original languageEnglish
Pages (from-to)941-947
Number of pages7
JournalThe Japanese Journal of Nephrology
Volume35
Issue number8
DOIs
Publication statusPublished - Jan 1 1993

Fingerprint

Collagen Type IV
Laminin
Proteoglycans
Glomerulonephritis
Extracellular Matrix
Fibronectins
Staining and Labeling
Membranoproliferative Glomerulonephritis
Lipoid Nephrosis
Membranous Glomerulonephritis
Indirect Fluorescent Antibody Technique
Immunoglobulin A
Kidney
Antibodies

Keywords

  • fibronectin
  • glomerulonephritis
  • heparansulfate proteoglycan-
  • laminin
  • type IV collagen

ASJC Scopus subject areas

  • Nephrology

Cite this

Alterations in glomerular extracellular matrix components in glomerulonephritis. / Ikeda, Shuji; Makino, Hirofumi; Ota, Zensuke.

In: The Japanese Journal of Nephrology, Vol. 35, No. 8, 01.01.1993, p. 941-947.

Research output: Contribution to journalArticle

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