Alterations in Ca2+-binding on plasma membrane after adaptation to salt stress of tobacco cells in suspension

Yoshiyuki Murata, Shinichi Katsura, Ichiro Obi, Tadaaki Kakutani

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15 Citations (Scopus)

Abstract

Electrophoresis was used to study effects of salinity on the characteristics of Ca2+ binding to the outer surface of plasma membrane (PM) of protoplasts isolated from two types of tobacco (Nicotiana tabacum L., cv. Bright Yellow) cultured cells that were adapted (tolerant) and unadapted (sensitive) to 50 mM NaCl stress. Electrophoretic analysis of salt-sensitive NaCl-unadapted cells shows that Na+ induced an appreciably higher degree of reduction in the amount of Ca2+ bound to PM compared with K+ with increasing concentration from 0.1 to 30 mM. In salt-tolerant NaCl-adapted cells, however, both Na+ and K+ ions induced almost the same degree of reduction in the amount of Ca2+ bound to PM in the physiological concentration range of Ca2+ in the medium between 2 and 4 mM. These results suggest that, under the physiological conditions, PM of salt-sensitive NaCl-unadapted cells has an appreciable amount of PM-bound Ca2+ that is desorbed much easier by Na+ than K+, whereas PM of salt-tolerant NaCl-adapted cells has the PM-bound Ca2+ that can be equally desorbed by Na+ and K+.

Original languageEnglish
Pages (from-to)1286-1292
Number of pages7
JournalPlant and Cell Physiology
Volume41
Issue number11
DOIs
Publication statusPublished - 2000

Keywords

  • Ca-binding
  • Nicotiana tabacum L.
  • Plasma membrane
  • Salt adaptation
  • Surface charge

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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