Alteration of odontoblast osteonectin expression following dental cavity preparation

Toshiyuki Itota, Yoshihiro Nishitani, Norio Sogawa, Chiharu Sogawa, Norifumi Konishi, Yasuhiro Torii

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cavity preparation can increase the active synthesis and secretion of non-collagenous proteins by odontoblasts, thus resulting in the deposition of tertiary dentine. In this study, the effect of cavity preparation on osteonectin expression was examined in odontoblasts of the rat tooth pulp. A class V cavity was prepared in rat first molars to stimulate odontoblastic secretory activity, and the animals were killed at various intervals. In the normal pulp, osteonectin immunoreactivity was detected in odontoblasts but not other cells. At 1 day after cavity preparation, immunoreactivity had diminished beneath the cavity. At 3 days, strong immunoreactivity could be detected in odontoblasts beneath the cavity. Numerous round cells underlying the odontoblastic layer also demonstrated immunoreactivity. Thereafter, the intensity of osteonectin immunoreactivity in odontoblasts beneath tertiary dentine decreased gradually, and at 30 and 60 days, it was weaker than in normal pulp. These findings suggest that osteonectin is actively synthesized by odontoblasts underlying a cavity in the initial stage of tertiary dentine formation.

Original languageEnglish
Pages (from-to)829-834
Number of pages6
JournalArchives of Oral Biology
Volume46
Issue number9
DOIs
Publication statusPublished - Aug 23 2001

Keywords

  • Cavity preparation
  • Immunohistochemistry
  • Odontoblast
  • Osteonectin
  • Tertiary dentine

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

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