Alteration of odontoblast osteonectin expression following dental cavity preparation

Toshiyuki Itota, Yoshihiro Nishitani, Norio Sogawa, Chiharu Sogawa, Norifumi Konishi, Yasuhiro Torii

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cavity preparation can increase the active synthesis and secretion of non-collagenous proteins by odontoblasts, thus resulting in the deposition of tertiary dentine. In this study, the effect of cavity preparation on osteonectin expression was examined in odontoblasts of the rat tooth pulp. A class V cavity was prepared in rat first molars to stimulate odontoblastic secretory activity, and the animals were killed at various intervals. In the normal pulp, osteonectin immunoreactivity was detected in odontoblasts but not other cells. At 1 day after cavity preparation, immunoreactivity had diminished beneath the cavity. At 3 days, strong immunoreactivity could be detected in odontoblasts beneath the cavity. Numerous round cells underlying the odontoblastic layer also demonstrated immunoreactivity. Thereafter, the intensity of osteonectin immunoreactivity in odontoblasts beneath tertiary dentine decreased gradually, and at 30 and 60 days, it was weaker than in normal pulp. These findings suggest that osteonectin is actively synthesized by odontoblasts underlying a cavity in the initial stage of tertiary dentine formation.

Original languageEnglish
Pages (from-to)829-834
Number of pages6
JournalArchives of Oral Biology
Volume46
Issue number9
DOIs
Publication statusPublished - 2001

Fingerprint

Dental Cavity Preparation
Osteonectin
Odontoblasts
Dentin
Tooth

Keywords

  • Cavity preparation
  • Immunohistochemistry
  • Odontoblast
  • Osteonectin
  • Tertiary dentine

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Alteration of odontoblast osteonectin expression following dental cavity preparation. / Itota, Toshiyuki; Nishitani, Yoshihiro; Sogawa, Norio; Sogawa, Chiharu; Konishi, Norifumi; Torii, Yasuhiro.

In: Archives of Oral Biology, Vol. 46, No. 9, 2001, p. 829-834.

Research output: Contribution to journalArticle

Itota, Toshiyuki ; Nishitani, Yoshihiro ; Sogawa, Norio ; Sogawa, Chiharu ; Konishi, Norifumi ; Torii, Yasuhiro. / Alteration of odontoblast osteonectin expression following dental cavity preparation. In: Archives of Oral Biology. 2001 ; Vol. 46, No. 9. pp. 829-834.
@article{f3a8469b61a84766b0fcf001d7f982c2,
title = "Alteration of odontoblast osteonectin expression following dental cavity preparation",
abstract = "Cavity preparation can increase the active synthesis and secretion of non-collagenous proteins by odontoblasts, thus resulting in the deposition of tertiary dentine. In this study, the effect of cavity preparation on osteonectin expression was examined in odontoblasts of the rat tooth pulp. A class V cavity was prepared in rat first molars to stimulate odontoblastic secretory activity, and the animals were killed at various intervals. In the normal pulp, osteonectin immunoreactivity was detected in odontoblasts but not other cells. At 1 day after cavity preparation, immunoreactivity had diminished beneath the cavity. At 3 days, strong immunoreactivity could be detected in odontoblasts beneath the cavity. Numerous round cells underlying the odontoblastic layer also demonstrated immunoreactivity. Thereafter, the intensity of osteonectin immunoreactivity in odontoblasts beneath tertiary dentine decreased gradually, and at 30 and 60 days, it was weaker than in normal pulp. These findings suggest that osteonectin is actively synthesized by odontoblasts underlying a cavity in the initial stage of tertiary dentine formation.",
keywords = "Cavity preparation, Immunohistochemistry, Odontoblast, Osteonectin, Tertiary dentine",
author = "Toshiyuki Itota and Yoshihiro Nishitani and Norio Sogawa and Chiharu Sogawa and Norifumi Konishi and Yasuhiro Torii",
year = "2001",
doi = "10.1016/S0003-9969(01)00041-3",
language = "English",
volume = "46",
pages = "829--834",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier Limited",
number = "9",

}

TY - JOUR

T1 - Alteration of odontoblast osteonectin expression following dental cavity preparation

AU - Itota, Toshiyuki

AU - Nishitani, Yoshihiro

AU - Sogawa, Norio

AU - Sogawa, Chiharu

AU - Konishi, Norifumi

AU - Torii, Yasuhiro

PY - 2001

Y1 - 2001

N2 - Cavity preparation can increase the active synthesis and secretion of non-collagenous proteins by odontoblasts, thus resulting in the deposition of tertiary dentine. In this study, the effect of cavity preparation on osteonectin expression was examined in odontoblasts of the rat tooth pulp. A class V cavity was prepared in rat first molars to stimulate odontoblastic secretory activity, and the animals were killed at various intervals. In the normal pulp, osteonectin immunoreactivity was detected in odontoblasts but not other cells. At 1 day after cavity preparation, immunoreactivity had diminished beneath the cavity. At 3 days, strong immunoreactivity could be detected in odontoblasts beneath the cavity. Numerous round cells underlying the odontoblastic layer also demonstrated immunoreactivity. Thereafter, the intensity of osteonectin immunoreactivity in odontoblasts beneath tertiary dentine decreased gradually, and at 30 and 60 days, it was weaker than in normal pulp. These findings suggest that osteonectin is actively synthesized by odontoblasts underlying a cavity in the initial stage of tertiary dentine formation.

AB - Cavity preparation can increase the active synthesis and secretion of non-collagenous proteins by odontoblasts, thus resulting in the deposition of tertiary dentine. In this study, the effect of cavity preparation on osteonectin expression was examined in odontoblasts of the rat tooth pulp. A class V cavity was prepared in rat first molars to stimulate odontoblastic secretory activity, and the animals were killed at various intervals. In the normal pulp, osteonectin immunoreactivity was detected in odontoblasts but not other cells. At 1 day after cavity preparation, immunoreactivity had diminished beneath the cavity. At 3 days, strong immunoreactivity could be detected in odontoblasts beneath the cavity. Numerous round cells underlying the odontoblastic layer also demonstrated immunoreactivity. Thereafter, the intensity of osteonectin immunoreactivity in odontoblasts beneath tertiary dentine decreased gradually, and at 30 and 60 days, it was weaker than in normal pulp. These findings suggest that osteonectin is actively synthesized by odontoblasts underlying a cavity in the initial stage of tertiary dentine formation.

KW - Cavity preparation

KW - Immunohistochemistry

KW - Odontoblast

KW - Osteonectin

KW - Tertiary dentine

UR - http://www.scopus.com/inward/record.url?scp=0034886914&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034886914&partnerID=8YFLogxK

U2 - 10.1016/S0003-9969(01)00041-3

DO - 10.1016/S0003-9969(01)00041-3

M3 - Article

C2 - 11420055

AN - SCOPUS:0034886914

VL - 46

SP - 829

EP - 834

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - 9

ER -