Alteration of glycan structures by swainsonine affects steroidogenesis in bovine luteal cells

Masahiro Sano, Kazuhisa Hashiba, Mai Higashi, Kiyoshi Okuda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The indolizidine alkaloid swainsonine (SW), a potent inhibitor of Golgi α-mannosidase II, is considered the primary toxicant in locoweeds causing toxicity syndrome known as locoism in livestock. Locoweed consumption decreases serum progesterone concentration and causes serious disorders in reproductive functions of livestock. However, direct effects of SW on luteal steroidogenic cells (LSCs) remain unclear. In the present study, we investigated the effect of N-glycan processing inhibition by SW on progesterone production and viability in cultured bovine LSCs. Swainsonine (0.1 μg/mL) fully inhibited glycosylation in the LSCs, but it had no effect on viability and progesterone production in the LSCs. Luteinizing hormone is known to promote progesterone production and expressions of steroidogenic acute regulatory protein and scavenger receptor type B class I. Pretreatment of LSCs with SW suppressed each of these effects, possibly by inhibiting LH receptor activity. These results suggest that SW inhibits N-glycan processing, which attenuates LH receptor activity, which, in turn, reduces progesterone production by CL.

Original languageEnglish
Pages (from-to)827-832
Number of pages6
JournalTheriogenology
Volume84
Issue number5
DOIs
Publication statusPublished - Sep 15 2015

Fingerprint

Swainsonine
swainsonine
Luteal Cells
luteal cells
steroidogenesis
Polysaccharides
polysaccharides
Progesterone
progesterone
cattle
LH Receptors
Livestock
receptors
indolizidine alkaloids
Indolizidines
livestock
mannosidases
viability
CD36 Antigens
reproductive disorders

Keywords

  • CL
  • LH
  • Locoweed
  • N-glycan
  • Progesterone

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Equine
  • Food Animals
  • Small Animals

Cite this

Alteration of glycan structures by swainsonine affects steroidogenesis in bovine luteal cells. / Sano, Masahiro; Hashiba, Kazuhisa; Higashi, Mai; Okuda, Kiyoshi.

In: Theriogenology, Vol. 84, No. 5, 15.09.2015, p. 827-832.

Research output: Contribution to journalArticle

Sano, Masahiro ; Hashiba, Kazuhisa ; Higashi, Mai ; Okuda, Kiyoshi. / Alteration of glycan structures by swainsonine affects steroidogenesis in bovine luteal cells. In: Theriogenology. 2015 ; Vol. 84, No. 5. pp. 827-832.
@article{aaa597abca89411387d50b7d1030a899,
title = "Alteration of glycan structures by swainsonine affects steroidogenesis in bovine luteal cells",
abstract = "The indolizidine alkaloid swainsonine (SW), a potent inhibitor of Golgi α-mannosidase II, is considered the primary toxicant in locoweeds causing toxicity syndrome known as locoism in livestock. Locoweed consumption decreases serum progesterone concentration and causes serious disorders in reproductive functions of livestock. However, direct effects of SW on luteal steroidogenic cells (LSCs) remain unclear. In the present study, we investigated the effect of N-glycan processing inhibition by SW on progesterone production and viability in cultured bovine LSCs. Swainsonine (0.1 μg/mL) fully inhibited glycosylation in the LSCs, but it had no effect on viability and progesterone production in the LSCs. Luteinizing hormone is known to promote progesterone production and expressions of steroidogenic acute regulatory protein and scavenger receptor type B class I. Pretreatment of LSCs with SW suppressed each of these effects, possibly by inhibiting LH receptor activity. These results suggest that SW inhibits N-glycan processing, which attenuates LH receptor activity, which, in turn, reduces progesterone production by CL.",
keywords = "CL, LH, Locoweed, N-glycan, Progesterone",
author = "Masahiro Sano and Kazuhisa Hashiba and Mai Higashi and Kiyoshi Okuda",
year = "2015",
month = "9",
day = "15",
doi = "10.1016/j.theriogenology.2015.05.026",
language = "English",
volume = "84",
pages = "827--832",
journal = "Theriogenology",
issn = "0093-691X",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Alteration of glycan structures by swainsonine affects steroidogenesis in bovine luteal cells

AU - Sano, Masahiro

AU - Hashiba, Kazuhisa

AU - Higashi, Mai

AU - Okuda, Kiyoshi

PY - 2015/9/15

Y1 - 2015/9/15

N2 - The indolizidine alkaloid swainsonine (SW), a potent inhibitor of Golgi α-mannosidase II, is considered the primary toxicant in locoweeds causing toxicity syndrome known as locoism in livestock. Locoweed consumption decreases serum progesterone concentration and causes serious disorders in reproductive functions of livestock. However, direct effects of SW on luteal steroidogenic cells (LSCs) remain unclear. In the present study, we investigated the effect of N-glycan processing inhibition by SW on progesterone production and viability in cultured bovine LSCs. Swainsonine (0.1 μg/mL) fully inhibited glycosylation in the LSCs, but it had no effect on viability and progesterone production in the LSCs. Luteinizing hormone is known to promote progesterone production and expressions of steroidogenic acute regulatory protein and scavenger receptor type B class I. Pretreatment of LSCs with SW suppressed each of these effects, possibly by inhibiting LH receptor activity. These results suggest that SW inhibits N-glycan processing, which attenuates LH receptor activity, which, in turn, reduces progesterone production by CL.

AB - The indolizidine alkaloid swainsonine (SW), a potent inhibitor of Golgi α-mannosidase II, is considered the primary toxicant in locoweeds causing toxicity syndrome known as locoism in livestock. Locoweed consumption decreases serum progesterone concentration and causes serious disorders in reproductive functions of livestock. However, direct effects of SW on luteal steroidogenic cells (LSCs) remain unclear. In the present study, we investigated the effect of N-glycan processing inhibition by SW on progesterone production and viability in cultured bovine LSCs. Swainsonine (0.1 μg/mL) fully inhibited glycosylation in the LSCs, but it had no effect on viability and progesterone production in the LSCs. Luteinizing hormone is known to promote progesterone production and expressions of steroidogenic acute regulatory protein and scavenger receptor type B class I. Pretreatment of LSCs with SW suppressed each of these effects, possibly by inhibiting LH receptor activity. These results suggest that SW inhibits N-glycan processing, which attenuates LH receptor activity, which, in turn, reduces progesterone production by CL.

KW - CL

KW - LH

KW - Locoweed

KW - N-glycan

KW - Progesterone

UR - http://www.scopus.com/inward/record.url?scp=84938972082&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938972082&partnerID=8YFLogxK

U2 - 10.1016/j.theriogenology.2015.05.026

DO - 10.1016/j.theriogenology.2015.05.026

M3 - Article

AN - SCOPUS:84938972082

VL - 84

SP - 827

EP - 832

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

IS - 5

ER -