Alteration of cytoskeletal molecules in a human T cell line caused by continuous exposure to chrysotile asbestos

Megumi Maeda, Ying Chen, Naoko Kumagai-Takei, Hiroaki Hayashi, Hidenori Matsuzaki, Suni Lee, Jun ichi Hiratsuka, Yasumitsu Nishimura, Yoshinobu Kimura, Takemi Otsuki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Among the various biological effects of asbestos such as fibrogenesis and carcinogenesis, we have been focusing on the immunological effects becausesilica (SiO2) and asbestos chemically is a mineral silicate of silica. Observations of the effects of asbestos on CD4+ T cells showed reduction of CXCR3 chemokine receptor and reduced capacity of interferon γ production. In particular, use of theHTLV-1 immortalized human T cell line, MT-2, and cDNA array analysis have helped to identify the modification of CXCR3. We investigated alteration of protein expression among MT-2 original cells that had no contact with asbestos, and six chrysotile-continuously exposed independent sublines using ProteinChip and two-dimensional gel electrophoresis (2DGE) assays. Further confirmation of the changes in protein expression due to asbestos exposure was obtained after the 2DGE method indicated protein modification of β-actin. β-actin was upregulated in mRNA, as were the levels of protein expression and phosphorylation. Moreover, a binding assay between cells and chrysotile showed that various molecules related to the cytoskeleton such as vimentin, myosin-9 and tubulin-β2, as well as β-actin, exhibited enhanced bindings in asbestos-exposed cells. The overall findings indicate that the cell surface cytoskeleton may play an important role in inducing the cellular changes caused by asbestos in immune cells, since fibers are not incorporated to the cells and how the alterations of cytoskeleton determined cell destiny to cause the reduction of tumor immunity is important to consider the biological effects of asbestos. Further studies to target several cytoskeleton-related molecules associated with the effects of asbestos will result in a better understanding of the immunological effects of asbestos and support the development of chemo-prevention to recover anti-tumor immunity in asbestos-exposed patients.

Original languageEnglish
Pages (from-to)1184-1191
Number of pages8
JournalImmunobiology
Volume218
Issue number9
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Serpentine Asbestos
Asbestos
T-Lymphocytes
Cell Line
Cytoskeleton
Actins
Immunity
Proteins
CXCR3 Receptors
Silicates
Protein Array Analysis
Chemokine Receptors
Electrophoresis, Gel, Two-Dimensional
Vimentin
Myosins
Tubulin
Oligonucleotide Array Sequence Analysis
Silicon Dioxide
Interferons
Minerals

Keywords

  • β-Actin
  • Asbestos
  • Cytoskeleton
  • Protein expression
  • T cell

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology

Cite this

Alteration of cytoskeletal molecules in a human T cell line caused by continuous exposure to chrysotile asbestos. / Maeda, Megumi; Chen, Ying; Kumagai-Takei, Naoko; Hayashi, Hiroaki; Matsuzaki, Hidenori; Lee, Suni; Hiratsuka, Jun ichi; Nishimura, Yasumitsu; Kimura, Yoshinobu; Otsuki, Takemi.

In: Immunobiology, Vol. 218, No. 9, 09.2013, p. 1184-1191.

Research output: Contribution to journalArticle

Maeda, M, Chen, Y, Kumagai-Takei, N, Hayashi, H, Matsuzaki, H, Lee, S, Hiratsuka, JI, Nishimura, Y, Kimura, Y & Otsuki, T 2013, 'Alteration of cytoskeletal molecules in a human T cell line caused by continuous exposure to chrysotile asbestos', Immunobiology, vol. 218, no. 9, pp. 1184-1191. https://doi.org/10.1016/j.imbio.2013.04.007
Maeda, Megumi ; Chen, Ying ; Kumagai-Takei, Naoko ; Hayashi, Hiroaki ; Matsuzaki, Hidenori ; Lee, Suni ; Hiratsuka, Jun ichi ; Nishimura, Yasumitsu ; Kimura, Yoshinobu ; Otsuki, Takemi. / Alteration of cytoskeletal molecules in a human T cell line caused by continuous exposure to chrysotile asbestos. In: Immunobiology. 2013 ; Vol. 218, No. 9. pp. 1184-1191.
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AU - Matsuzaki, Hidenori

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AU - Hiratsuka, Jun ichi

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