TY - JOUR
T1 - Alteration of chemokine production in bovine endometrial epithelial and stromal cells under heat stress conditions
AU - Sakai, Shunsuke
AU - Hatabu, Toshimitsu
AU - Yamamoto, Yuki
AU - Kimura, Koji
N1 - Funding Information:
We greatly appreciate Okayama Meat Center (Okayama Japan) and Tsuyama Meat Center (Tsuyama Japan) for providing the bovine uteri and Dr. Shigeki Inumaru (National Agriculture and Food Research Organization, Japan) for providing bovine IFN?. We thank Mr. Takashi Nokubo and Mr. Tetsuya Aoyama (Tsudaka Livestock Farm, Okayama Univ., Japan) for their technical assistance and maintenance of experimental animals. We also thank Maho Munetomo (Laboratory of Reproductive Physiology, Okayama Univ.) for her illustration.
Publisher Copyright:
© 2020 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society
PY - 2020/11
Y1 - 2020/11
N2 - After parturition, cows frequently develop uterine bacterial infections, resulting in the onset of endometritis. To eliminate the bacteria, bovine endometrial cells secrete chemokines, such as IL-6 and MCP1, which attract macrophages (MΦs) to the subepithelial stroma. These attracted MΦs are not only involved in bacterial elimination but also the orchestration of inflammation and tissue repair. These immune responses aid in the recovery from endometritis; however, the recovery from endometritis takes longer in summer than in any other season. Based on these findings, we hypothesized that heat stress (HS) affects the chemokine production in endometrial cells. To confirm this hypothesis, we compared IL-6 and MCP1 production induced by lipopolysaccharide (LPS) in bovine endometrial epithelial and stromal cells under normal (38.5°C) and HS conditions (40.5°C). In the endometrial epithelial cells, IL-6 production stimulated by LPS was significantly (p <.05) suppressed under HS conditions. MCP1 production in endometrial epithelial cells was not detected under both the control and HS conditions regardless of the presence of LPS. Moreover, LPS significantly (p <.05) stimulated IL-6 and MCP1 production in endometrial stromal cells. Moreover, HS significantly (p <.05) enhanced their production compared to that under the control conditions. In addition, HS did not affect the migration ability of MΦs; however, the supernatant of the endometrial stromal cells cultured under the HS condition significantly (p <.05) attracted the MΦs when compared to the control condition. These results suggest that HS disrupts chemokine production in two types of endometrial cells and alters the distribution of MΦs in the endometrium during the summer.
AB - After parturition, cows frequently develop uterine bacterial infections, resulting in the onset of endometritis. To eliminate the bacteria, bovine endometrial cells secrete chemokines, such as IL-6 and MCP1, which attract macrophages (MΦs) to the subepithelial stroma. These attracted MΦs are not only involved in bacterial elimination but also the orchestration of inflammation and tissue repair. These immune responses aid in the recovery from endometritis; however, the recovery from endometritis takes longer in summer than in any other season. Based on these findings, we hypothesized that heat stress (HS) affects the chemokine production in endometrial cells. To confirm this hypothesis, we compared IL-6 and MCP1 production induced by lipopolysaccharide (LPS) in bovine endometrial epithelial and stromal cells under normal (38.5°C) and HS conditions (40.5°C). In the endometrial epithelial cells, IL-6 production stimulated by LPS was significantly (p <.05) suppressed under HS conditions. MCP1 production in endometrial epithelial cells was not detected under both the control and HS conditions regardless of the presence of LPS. Moreover, LPS significantly (p <.05) stimulated IL-6 and MCP1 production in endometrial stromal cells. Moreover, HS significantly (p <.05) enhanced their production compared to that under the control conditions. In addition, HS did not affect the migration ability of MΦs; however, the supernatant of the endometrial stromal cells cultured under the HS condition significantly (p <.05) attracted the MΦs when compared to the control condition. These results suggest that HS disrupts chemokine production in two types of endometrial cells and alters the distribution of MΦs in the endometrium during the summer.
KW - chemokine
KW - cow
KW - endometrial cells
KW - endometritis
KW - heat stress
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U2 - 10.14814/phy2.14640
DO - 10.14814/phy2.14640
M3 - Article
C2 - 33230953
AN - SCOPUS:85096700260
VL - 8
JO - Physiological Reports
JF - Physiological Reports
SN - 2051-817X
IS - 22
M1 - e14640
ER -