Alteration of chemokine production in bovine endometrial epithelial and stromal cells under heat stress conditions

Shunsuke Sakai, Toshimitsu Hatabu, Yuki Yamamoto, Koji Kimura

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


After parturition, cows frequently develop uterine bacterial infections, resulting in the onset of endometritis. To eliminate the bacteria, bovine endometrial cells secrete chemokines, such as IL-6 and MCP1, which attract macrophages (MΦs) to the subepithelial stroma. These attracted MΦs are not only involved in bacterial elimination but also the orchestration of inflammation and tissue repair. These immune responses aid in the recovery from endometritis; however, the recovery from endometritis takes longer in summer than in any other season. Based on these findings, we hypothesized that heat stress (HS) affects the chemokine production in endometrial cells. To confirm this hypothesis, we compared IL-6 and MCP1 production induced by lipopolysaccharide (LPS) in bovine endometrial epithelial and stromal cells under normal (38.5°C) and HS conditions (40.5°C). In the endometrial epithelial cells, IL-6 production stimulated by LPS was significantly (p <.05) suppressed under HS conditions. MCP1 production in endometrial epithelial cells was not detected under both the control and HS conditions regardless of the presence of LPS. Moreover, LPS significantly (p <.05) stimulated IL-6 and MCP1 production in endometrial stromal cells. Moreover, HS significantly (p <.05) enhanced their production compared to that under the control conditions. In addition, HS did not affect the migration ability of MΦs; however, the supernatant of the endometrial stromal cells cultured under the HS condition significantly (p <.05) attracted the MΦs when compared to the control condition. These results suggest that HS disrupts chemokine production in two types of endometrial cells and alters the distribution of MΦs in the endometrium during the summer.

Original languageEnglish
Article numbere14640
JournalPhysiological Reports
Issue number22
Publication statusPublished - Nov 2020


  • chemokine
  • cow
  • endometrial cells
  • endometritis
  • heat stress

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


Dive into the research topics of 'Alteration of chemokine production in bovine endometrial epithelial and stromal cells under heat stress conditions'. Together they form a unique fingerprint.

Cite this