TY - JOUR
T1 - Alteration in plasma free amino acid levels and its association with gout
AU - Mahbub, M. H.
AU - Yamaguchi, Natsu
AU - Takahashi, Hidekazu
AU - Hase, Ryosuke
AU - Amano, Hiroki
AU - Kobayashi-Miura, Mikiko
AU - Kanda, Hideyuki
AU - Fujita, Yasuyuki
AU - Yamamoto, Hiroshi
AU - Yamamoto, Mai
AU - Kikuchi, Shinya
AU - Ikeda, Atsuko
AU - Kageyama, Naoko
AU - Nakamura, Mina
AU - Ishimaru, Yasutaka
AU - Sunagawa, Hiroshi
AU - Tanabe, Tsuyoshi
N1 - Funding Information:
HY, MY, SK, AI, NK, MN are employees of Ajinomoto Co., Inc. TT, HA, and YF received research grants from Ajinomoto Co., Inc. This does not alter the authors’ adherences to all of the journal policies. The authors declare that they have no competing interests.
Publisher Copyright:
© The Author(s).
PY - 2017
Y1 - 2017
N2 - Background: Studies on the association of plasma-free amino acids with gout are very limited and produced conflicting results. Therefore, we sought to explore and characterize the plasma-free amino acid (PFAA) profile in patients with gout and evaluate its association with the latter. Methods: Data from a total of 819 subjects (including 34 patients with gout) undergoing an annual health examination program in Shimane, Japan were considered for this study. Venous blood samples were collected from the subjects and concentrations of 19 plasma amino acids were determined by high-performance liquid chromatography-electrospray ionization-mass spectrometry. Student's t-test was applied for comparison of variables between patient and control groups. The relationships between the presence or absence of gout and individual amino acids were investigated by logistic regression analysis controlling for the effects of potential demographic confounders. Results: Among 19 amino acids, the levels of 10 amino acids (alanine, glycine, isoleucine, leucine, methionine, phenylalanine, proline, serine, tryptophan, valine) differed significantly (P < .001 to .05) between the patient and control groups. Univariate logistic regression analysis revealed that plasma levels of alanine, isoleucine, leucine, phenylalanine, tryptophan and valine had significant positive associations (P < .005 to .05) whereas glycine and serine had significant inverse association (P < .05) with gout. Conclusions: The observed significant changes in PFAA profiles may have important implications for improving our understanding of pathophysiology, diagnosis and prevention of gout. The findings of this study need further confirmation in future large-scale studies involving a larger number of patients with gout.
AB - Background: Studies on the association of plasma-free amino acids with gout are very limited and produced conflicting results. Therefore, we sought to explore and characterize the plasma-free amino acid (PFAA) profile in patients with gout and evaluate its association with the latter. Methods: Data from a total of 819 subjects (including 34 patients with gout) undergoing an annual health examination program in Shimane, Japan were considered for this study. Venous blood samples were collected from the subjects and concentrations of 19 plasma amino acids were determined by high-performance liquid chromatography-electrospray ionization-mass spectrometry. Student's t-test was applied for comparison of variables between patient and control groups. The relationships between the presence or absence of gout and individual amino acids were investigated by logistic regression analysis controlling for the effects of potential demographic confounders. Results: Among 19 amino acids, the levels of 10 amino acids (alanine, glycine, isoleucine, leucine, methionine, phenylalanine, proline, serine, tryptophan, valine) differed significantly (P < .001 to .05) between the patient and control groups. Univariate logistic regression analysis revealed that plasma levels of alanine, isoleucine, leucine, phenylalanine, tryptophan and valine had significant positive associations (P < .005 to .05) whereas glycine and serine had significant inverse association (P < .05) with gout. Conclusions: The observed significant changes in PFAA profiles may have important implications for improving our understanding of pathophysiology, diagnosis and prevention of gout. The findings of this study need further confirmation in future large-scale studies involving a larger number of patients with gout.
KW - Amino acids
KW - Gout
KW - Plasma
KW - Profile
KW - Relationship
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U2 - 10.1186/s12199-017-0609-8
DO - 10.1186/s12199-017-0609-8
M3 - Article
C2 - 29165113
AN - SCOPUS:85031744998
VL - 22
JO - Environmental Health and Preventive Medicine
JF - Environmental Health and Preventive Medicine
SN - 1342-078X
IS - 1
ER -
