Alpinia zerumbet (Pers.): Food and medicinal plant with potential in vitro and in vivo anti-cancer activities

Maram Hussein Zahra; Tarek A.R. Salem; Bishoy El-Aarag; Nermeen Yosri; Samah EL-Ghlban; Kholoud Zaki; Amel H. Marei; Aida Abd El-Wahed; Aamer Saeed; Alfi Khatib; Mohamed F. AlAjmi; Abdulrahman M. Shathili; Jianbo Xiao; Shaden A.M. Khalifa; Hesham R. El-Seedi

doi:10.3390/molecules24132495

Alpinia zerumbet (Pers.): Food and medicinal plant with potential in vitro and in vivo anti-cancer activities

Maram Hussein Zahra, Tarek A.R. Salem, Bishoy El-Aarag, Nermeen Yosri, Samah EL-Ghlban, Kholoud Zaki, Amel H. Marei, Aida Abd El-Wahed, Aamer Saeed, Alfi Khatib, Mohamed F. AlAjmi, Abdulrahman M. Shathili, Jianbo Xiao, Shaden A.M. Khalifa, Hesham R. El-Seedi

Graduate School of Interdisciplinary Science and Engineering in Health Systems

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Background/Aim: Plants play an important role in anti-cancer drug discovery, therefore, the current study aimed to evaluate the biological activity of Alpinia zerumbet (A. zerumbet) flowers. Methods: The phytochemical and biological criteria of A. zerumbet were in vitro investigated as well as in mouse xenograft model. Results: A. zerumbet extracts, specially CH₂Cl₂ and MeOH extracts, exhibited the highest potent anti-tumor activity against Ehrlich ascites carcinoma (EAC) cells. The most active CH₂Cl₂ extract was subjected to bioassay-guided fractionation leading to isolatation of the naturally occurring 5,6-dehydrokawain (DK) which was characterized by IR, MS, ¹H-NMR and ¹³C-NMR. A. zerumbet extracts, specially MeOH and CH₂Cl₂ extracts, exhibited significant inhibitory activity towards tumor volume (TV). Furthermore, A. zerumbet extracts declined the high level of malonaldehyde (MDA) as well as elevated the levels of superoxide dismutase (SOD) and catalase (CAT) in liver tissue homogenate. Moreover, DK showed anti-proliferative action on different human cancer cell lines. The recorded IC₅₀ values against breast carcinoma (MCF-7), liver carcinoma (Hep-G2) and larynx carcinoma cells (HEP-2) were 3.08, 6.8, and 8.7 µg/mL, respectively. Conclusion: Taken together, these findings open the door for further investigations in order to explore the potential medicinal properties of A. zerumbet.

Original language	English
Article number	2495
Journal	Molecules
Volume	24
Issue number	13
DOIs	https://doi.org/10.3390/molecules24132495
Publication status	Published - 2019

Keywords

5,6-dehydrokawain
Alpinia zerumbet
Anti-oxidant
Anti-tumor
Ehrlich ascites carcinoma

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

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Zahra, M. H., Salem, T. A. R., El-Aarag, B., Yosri, N., EL-Ghlban, S., Zaki, K., Marei, A. H., El-Wahed, A. A., Saeed, A., Khatib, A., AlAjmi, M. F., Shathili, A. M., Xiao, J., Khalifa, S. A. M., & El-Seedi, H. R. (2019). Alpinia zerumbet (Pers.): Food and medicinal plant with potential in vitro and in vivo anti-cancer activities. Molecules, 24(13), [2495]. https://doi.org/10.3390/molecules24132495

Alpinia zerumbet (Pers.) : Food and medicinal plant with potential in vitro and in vivo anti-cancer activities. / Zahra, Maram Hussein; Salem, Tarek A.R.; El-Aarag, Bishoy; Yosri, Nermeen; EL-Ghlban, Samah; Zaki, Kholoud; Marei, Amel H.; El-Wahed, Aida Abd; Saeed, Aamer; Khatib, Alfi; AlAjmi, Mohamed F.; Shathili, Abdulrahman M.; Xiao, Jianbo; Khalifa, Shaden A.M.; El-Seedi, Hesham R.

In: Molecules, Vol. 24, No. 13, 2495, 2019.

Research output: Contribution to journal › Article › peer-review

Zahra, MH, Salem, TAR, El-Aarag, B, Yosri, N, EL-Ghlban, S, Zaki, K, Marei, AH, El-Wahed, AA, Saeed, A, Khatib, A, AlAjmi, MF, Shathili, AM, Xiao, J, Khalifa, SAM & El-Seedi, HR 2019, 'Alpinia zerumbet (Pers.): Food and medicinal plant with potential in vitro and in vivo anti-cancer activities', Molecules, vol. 24, no. 13, 2495. https://doi.org/10.3390/molecules24132495

Zahra, Maram Hussein ; Salem, Tarek A.R. ; El-Aarag, Bishoy ; Yosri, Nermeen ; EL-Ghlban, Samah ; Zaki, Kholoud ; Marei, Amel H. ; El-Wahed, Aida Abd ; Saeed, Aamer ; Khatib, Alfi ; AlAjmi, Mohamed F. ; Shathili, Abdulrahman M. ; Xiao, Jianbo ; Khalifa, Shaden A.M. ; El-Seedi, Hesham R. / Alpinia zerumbet (Pers.) : Food and medicinal plant with potential in vitro and in vivo anti-cancer activities. In: Molecules. 2019 ; Vol. 24, No. 13.

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title = "Alpinia zerumbet (Pers.): Food and medicinal plant with potential in vitro and in vivo anti-cancer activities",

abstract = "Background/Aim: Plants play an important role in anti-cancer drug discovery, therefore, the current study aimed to evaluate the biological activity of Alpinia zerumbet (A. zerumbet) flowers. Methods: The phytochemical and biological criteria of A. zerumbet were in vitro investigated as well as in mouse xenograft model. Results: A. zerumbet extracts, specially CH2Cl2 and MeOH extracts, exhibited the highest potent anti-tumor activity against Ehrlich ascites carcinoma (EAC) cells. The most active CH2Cl2 extract was subjected to bioassay-guided fractionation leading to isolatation of the naturally occurring 5,6-dehydrokawain (DK) which was characterized by IR, MS, 1H-NMR and 13C-NMR. A. zerumbet extracts, specially MeOH and CH2Cl2 extracts, exhibited significant inhibitory activity towards tumor volume (TV). Furthermore, A. zerumbet extracts declined the high level of malonaldehyde (MDA) as well as elevated the levels of superoxide dismutase (SOD) and catalase (CAT) in liver tissue homogenate. Moreover, DK showed anti-proliferative action on different human cancer cell lines. The recorded IC50 values against breast carcinoma (MCF-7), liver carcinoma (Hep-G2) and larynx carcinoma cells (HEP-2) were 3.08, 6.8, and 8.7 µg/mL, respectively. Conclusion: Taken together, these findings open the door for further investigations in order to explore the potential medicinal properties of A. zerumbet.",

keywords = "5,6-dehydrokawain, Alpinia zerumbet, Anti-oxidant, Anti-tumor, Ehrlich ascites carcinoma",

author = "Zahra, {Maram Hussein} and Salem, {Tarek A.R.} and Bishoy El-Aarag and Nermeen Yosri and Samah EL-Ghlban and Kholoud Zaki and Marei, {Amel H.} and El-Wahed, {Aida Abd} and Aamer Saeed and Alfi Khatib and AlAjmi, {Mohamed F.} and Shathili, {Abdulrahman M.} and Jianbo Xiao and Khalifa, {Shaden A.M.} and El-Seedi, {Hesham R.}",

note = "Funding Information: Funding: The authors wish to express their appreciation to the International Scientific Partnership Program (ISPP) at King Saud University for funding this research work through an ISPP-126 grant awarded to H.R.E. and M.F.E. H.R.E. Alfi Khatib wish to thank the International Islamic University Malaysia for the Publication Research Initiative Grant Fund (PRIGS18-027-0027). wishes to thank the Swedish Research links grant 2016-05885 (VR for the years 2017–2019) for financial support.",

year = "2019",

doi = "10.3390/molecules24132495",

language = "English",

volume = "24",

journal = "Molecules",

issn = "1420-3049",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "13",

}

TY - JOUR

T1 - Alpinia zerumbet (Pers.)

T2 - Food and medicinal plant with potential in vitro and in vivo anti-cancer activities

AU - Zahra, Maram Hussein

AU - Salem, Tarek A.R.

AU - El-Aarag, Bishoy

AU - Yosri, Nermeen

AU - EL-Ghlban, Samah

AU - Zaki, Kholoud

AU - Marei, Amel H.

AU - El-Wahed, Aida Abd

AU - Saeed, Aamer

AU - Khatib, Alfi

AU - AlAjmi, Mohamed F.

AU - Shathili, Abdulrahman M.

AU - Xiao, Jianbo

AU - Khalifa, Shaden A.M.

AU - El-Seedi, Hesham R.

N1 - Funding Information: Funding: The authors wish to express their appreciation to the International Scientific Partnership Program (ISPP) at King Saud University for funding this research work through an ISPP-126 grant awarded to H.R.E. and M.F.E. H.R.E. Alfi Khatib wish to thank the International Islamic University Malaysia for the Publication Research Initiative Grant Fund (PRIGS18-027-0027). wishes to thank the Swedish Research links grant 2016-05885 (VR for the years 2017–2019) for financial support.

PY - 2019

Y1 - 2019

N2 - Background/Aim: Plants play an important role in anti-cancer drug discovery, therefore, the current study aimed to evaluate the biological activity of Alpinia zerumbet (A. zerumbet) flowers. Methods: The phytochemical and biological criteria of A. zerumbet were in vitro investigated as well as in mouse xenograft model. Results: A. zerumbet extracts, specially CH2Cl2 and MeOH extracts, exhibited the highest potent anti-tumor activity against Ehrlich ascites carcinoma (EAC) cells. The most active CH2Cl2 extract was subjected to bioassay-guided fractionation leading to isolatation of the naturally occurring 5,6-dehydrokawain (DK) which was characterized by IR, MS, 1H-NMR and 13C-NMR. A. zerumbet extracts, specially MeOH and CH2Cl2 extracts, exhibited significant inhibitory activity towards tumor volume (TV). Furthermore, A. zerumbet extracts declined the high level of malonaldehyde (MDA) as well as elevated the levels of superoxide dismutase (SOD) and catalase (CAT) in liver tissue homogenate. Moreover, DK showed anti-proliferative action on different human cancer cell lines. The recorded IC50 values against breast carcinoma (MCF-7), liver carcinoma (Hep-G2) and larynx carcinoma cells (HEP-2) were 3.08, 6.8, and 8.7 µg/mL, respectively. Conclusion: Taken together, these findings open the door for further investigations in order to explore the potential medicinal properties of A. zerumbet.

AB - Background/Aim: Plants play an important role in anti-cancer drug discovery, therefore, the current study aimed to evaluate the biological activity of Alpinia zerumbet (A. zerumbet) flowers. Methods: The phytochemical and biological criteria of A. zerumbet were in vitro investigated as well as in mouse xenograft model. Results: A. zerumbet extracts, specially CH2Cl2 and MeOH extracts, exhibited the highest potent anti-tumor activity against Ehrlich ascites carcinoma (EAC) cells. The most active CH2Cl2 extract was subjected to bioassay-guided fractionation leading to isolatation of the naturally occurring 5,6-dehydrokawain (DK) which was characterized by IR, MS, 1H-NMR and 13C-NMR. A. zerumbet extracts, specially MeOH and CH2Cl2 extracts, exhibited significant inhibitory activity towards tumor volume (TV). Furthermore, A. zerumbet extracts declined the high level of malonaldehyde (MDA) as well as elevated the levels of superoxide dismutase (SOD) and catalase (CAT) in liver tissue homogenate. Moreover, DK showed anti-proliferative action on different human cancer cell lines. The recorded IC50 values against breast carcinoma (MCF-7), liver carcinoma (Hep-G2) and larynx carcinoma cells (HEP-2) were 3.08, 6.8, and 8.7 µg/mL, respectively. Conclusion: Taken together, these findings open the door for further investigations in order to explore the potential medicinal properties of A. zerumbet.

KW - 5,6-dehydrokawain

KW - Alpinia zerumbet

KW - Anti-oxidant

KW - Anti-tumor

KW - Ehrlich ascites carcinoma

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DO - 10.3390/molecules24132495

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