Allyl isothiocyanate induces stomatal closure in Vicia faba

Muhammad Abdus Sobahan, Nasima Akter, Eiji Okuma, Misugi Uraji, Wenxiu Ye, Izumi Mori, Yoshimasa Nakamura, Yoshiyuki Murata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Isothiocyanates are enzymatically produced from glucosinolates in plants, and allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis thaliana. In this study, we investigated stomatal responses to AITC in Vicia faba. AITC-induced stomatal closure accompanied by reactive oxygen species (ROS) and NO production, cytosolic alkalization and glutathione (GSH) depletion in V. faba. GSH monoethyl ester induced stomatal reopening and suppressed AITC-induced GSH depletion in guard cells. Exogenous catalase and a peroxidase inhibitor, salicylhydroxamic acid, inhibited AITC-induced stomatal closure, unlike an NAD(P)H oxidase inhibitor, diphenylene iodonium chloride. The peroxidase inhibitor also abolished the AITC-induced ROS production, NO production, and cytosolic alkalization. AITC-induced stomatal closure was suppressed by an NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5- Tetramethylimidazoline-1-oxyl-3-oxide, and an agent to acidify cytosol, butyrate. These results indicate that AITC-induced stomatal closure in V. faba as well as in A. thaliana and suggest that AITC signaling in guard cells is conserved in both plants.

Original languageEnglish
Pages (from-to)1737-1742
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume79
Issue number10
DOIs
Publication statusPublished - 2015

Fingerprint

Vicia faba
Arabidopsis
Peroxidase
Reactive Oxygen Species
Isothiocyanates
Glucosinolates
allyl isothiocyanate
Butyrates
NADPH Oxidase
Cytosol
Catalase
Glutathione
Chlorides
Esters

Keywords

  • Guard cells
  • Peroxidase
  • Signaling
  • Stomatal closure

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Allyl isothiocyanate induces stomatal closure in Vicia faba. / Sobahan, Muhammad Abdus; Akter, Nasima; Okuma, Eiji; Uraji, Misugi; Ye, Wenxiu; Mori, Izumi; Nakamura, Yoshimasa; Murata, Yoshiyuki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 79, No. 10, 2015, p. 1737-1742.

Research output: Contribution to journalArticle

Sobahan, Muhammad Abdus ; Akter, Nasima ; Okuma, Eiji ; Uraji, Misugi ; Ye, Wenxiu ; Mori, Izumi ; Nakamura, Yoshimasa ; Murata, Yoshiyuki. / Allyl isothiocyanate induces stomatal closure in Vicia faba. In: Bioscience, Biotechnology and Biochemistry. 2015 ; Vol. 79, No. 10. pp. 1737-1742.
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