Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis

Md Atiqur Rahman Khokon, Md Sarwar Jahan, Taniya Rahman, Mohammad Anowar Hossain, Daichi Muroyama, Ikuko Minami, Shintaro Munemasa, Izumi C. Mori, Yoshimasa Nakamura, Yoshiyuki Murata

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure in Arabidopsis via production of reactive oxygen species (ROS) and nitric oxide (NO), and elevation of cytosolic Ca 2+. AITC-induced stomatal closures were partially inhibited by an inhibitor of NADPH oxidase and completely inhibited by glutathione monoethyl ester (GSHmee). AITC-induced stomatal closure and ROS production were examined in abscisic acid (ABA) deficient mutant aba2-2 and methyl jasmonate (MeJA)-deficient mutant aos to elucidate involvement of endogenous ABA and MeJA. Genetic evidences have demonstrated that AITC-induced stomatal closure required MeJA priming but not ABA priming. These results raise the possibility that crucifer plants produce ITCs to induce stomatal closure, leading to suppression of water loss and invasion of fungi through stomata.

Original languageEnglish
Pages (from-to)1900-1906
Number of pages7
JournalPlant, Cell and Environment
Volume34
Issue number11
DOIs
Publication statusPublished - Nov 1 2011

Keywords

  • Glutathione
  • Myrosinases
  • NO
  • ROS
  • [Ca ] oscillation

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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