Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis

Md Atiqur Rahman Khokon, Md Sarwar Jahan, Taniya Rahman, Mohammad Anowar Hossain, Daichi Muroyama, Ikuko Minami, Shintaro Munemasa, Izumi Mori, Yoshimasa Nakamura, Yoshiyuki Murata

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Isothiocyanates (ITCs) are degradation products of glucosinolates in crucifer plants and have repellent effect on insects, pathogens and herbivores. In this study, we report that exogenously applied allyl isothiocyanate (AITC) induced stomatal closure in Arabidopsis via production of reactive oxygen species (ROS) and nitric oxide (NO), and elevation of cytosolic Ca 2+. AITC-induced stomatal closures were partially inhibited by an inhibitor of NADPH oxidase and completely inhibited by glutathione monoethyl ester (GSHmee). AITC-induced stomatal closure and ROS production were examined in abscisic acid (ABA) deficient mutant aba2-2 and methyl jasmonate (MeJA)-deficient mutant aos to elucidate involvement of endogenous ABA and MeJA. Genetic evidences have demonstrated that AITC-induced stomatal closure required MeJA priming but not ABA priming. These results raise the possibility that crucifer plants produce ITCs to induce stomatal closure, leading to suppression of water loss and invasion of fungi through stomata.

Original languageEnglish
Pages (from-to)1900-1906
Number of pages7
JournalPlant, Cell and Environment
Volume34
Issue number11
DOIs
Publication statusPublished - Nov 2011

Fingerprint

allyl isothiocyanate
Arabidopsis
Abscisic Acid
methyl jasmonate
Isothiocyanates
abscisic acid
isothiocyanates
reactive oxygen species
Reactive Oxygen Species
Glucosinolates
mutants
Herbivory
entomopathogens
NADPH Oxidase
glucosinolates
repellents
stomata
nitric oxide
Insects
glutathione

Keywords

  • [Ca ] oscillation
  • Glutathione
  • Myrosinases
  • NO
  • ROS

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Khokon, M. A. R., Jahan, M. S., Rahman, T., Hossain, M. A., Muroyama, D., Minami, I., ... Murata, Y. (2011). Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis. Plant, Cell and Environment, 34(11), 1900-1906. https://doi.org/10.1111/j.1365-3040.2011.02385.x

Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis. / Khokon, Md Atiqur Rahman; Jahan, Md Sarwar; Rahman, Taniya; Hossain, Mohammad Anowar; Muroyama, Daichi; Minami, Ikuko; Munemasa, Shintaro; Mori, Izumi; Nakamura, Yoshimasa; Murata, Yoshiyuki.

In: Plant, Cell and Environment, Vol. 34, No. 11, 11.2011, p. 1900-1906.

Research output: Contribution to journalArticle

Khokon MAR, Jahan MS, Rahman T, Hossain MA, Muroyama D, Minami I et al. Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis. Plant, Cell and Environment. 2011 Nov;34(11):1900-1906. https://doi.org/10.1111/j.1365-3040.2011.02385.x
Khokon, Md Atiqur Rahman ; Jahan, Md Sarwar ; Rahman, Taniya ; Hossain, Mohammad Anowar ; Muroyama, Daichi ; Minami, Ikuko ; Munemasa, Shintaro ; Mori, Izumi ; Nakamura, Yoshimasa ; Murata, Yoshiyuki. / Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis. In: Plant, Cell and Environment. 2011 ; Vol. 34, No. 11. pp. 1900-1906.
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