More than 90% of IL-2-activated plastic-adherent murine splenocytes (A- LAK4 cells) are NK1.1+ NK cells anti both H-2(b/b) homozygous C57BL/6- and H-2(b/d) heterozygous (C57BL/6 x DBA/2)F1 (B6D2F1)-derived populations of such cells contain an Ly-49A+ subset. In B6D2F1 A-LAK cells, as well as in freshly isolated spleen cells of the same mice, Ly-49A+ cells represent approximately 10% of NK1.1+ cells. However, the level of Ly-49A expression in B6D2F1 NK cells is lower than in C57BL/6 (B6). The cytolytic activity of B6- and B6D2F1-derived A-LAK cells against normal target cells is specific, and is in agreement with the known patterns of natural resistance in vivo against Hh-1-mismatched bone marrow allografts. H-2(b) lymphoma cells transfected with the D(d) gene, but not the L(d) gene, no longer express the Hh-1(b) phenotype that is recognized by B6D2F1 A LAK cells, raising the possibility that this selective effect of the D(d) gene on Hh-1(b) phenotype is related to the known inability of Ly-49A+ A-LAK cells to kill D(d)- expressing tumor target cells. Depletion of Ly-49A+ A-LAK cells by Ab and complement reduces the lytic capacity of B6D2F1 A-LAK cells against normal B6 target cells of the Hh-1(b) phenotype to one-third of the original level. Conversely, positively selected Ly-49A+ A-LAK cells are enriched for the same activity. Tire results, therefore, favor tire view that the Hh-1 phenotype of the target cells may be defined largely by the effector cell's recognition of class I Ags on the target cell surface.
|Number of pages||11|
|Journal||Journal of Immunology|
|Publication status||Published - 1995|
ASJC Scopus subject areas
- Immunology and Allergy