Allergic airway inflammation by nasal inoculation of particulate matter (PM2.5) in NC/Nga mice

Keiki Ogino, Ran Zhang, Hidekazu Takahashi, Kei Takemoto, Masayuki Kubo, Ikuo Murakami, Da Hong Wang, Yoshihisa Fujikura

Research output: Contribution to journalArticle

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Abstract

To evaluate the effect of airborne particulate matter 2.5 (PM 2.5) in winter on airway inflammation, water-soluble supernatant (Sup) and water-insoluble precipitate (Pre) in PM2.5 were inoculated in NC/Nga mice with high sensitivity to mite allergens. Sup with aluminum oxide was injected intraperitoneally for sensitization. Five days later, Sup, Pre or both Sup and Pre were inoculated via the nasal route five times for more sensitization and a challenge inoculation on the 11th day in NC/Nga mice. On the 12th day, mice were examined for airway hyperresponsiveness (AHR), BALF cell count and IL-1β concentration, mRNA expression of Th1 and Th2 cytokines, chemokines such as eotaxin 1 and eotaxin 2, inflammasomal complex molecules such as IL-1β, caspase 1 and the nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) in lung tissue as well as histopathology. The synergistic effect of Sup and Pre was observed in terms of increases in AHR, BALF cells, the mRNA expression of IL-13, eotaxin1 and IL-1β, and the IL-1β concentration in BALF. Intracellular deposits of insoluble particulates were observed in macrophages around inflammatory granulation of the mouse group treated with Sup and Pre. These results suggest that PM2.5 can induce airway hyperresponsiveness in mice with genetically high sensitivity to mite allergens by an inflammasome-associated mechanism and synergistic action of insoluble particulates and soluble components.

Original languageEnglish
Article numbere92710
JournalPLoS One
Volume9
Issue number3
DOIs
Publication statusPublished - Mar 26 2014

Fingerprint

Particulate Matter
Nose
interleukin-1
Precipitates
particulates
Interleukin-1
inflammation
Inflammation
mice
Mites
allergens
Allergens
mites
Chemokine CCL24
Chemokine CCL11
caspase-1
Inflammasomes
Caspase 1
Messenger RNA
Granulation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ogino, K., Zhang, R., Takahashi, H., Takemoto, K., Kubo, M., Murakami, I., ... Fujikura, Y. (2014). Allergic airway inflammation by nasal inoculation of particulate matter (PM2.5) in NC/Nga mice. PLoS One, 9(3), [e92710]. https://doi.org/10.1371/journal.pone.0092710

Allergic airway inflammation by nasal inoculation of particulate matter (PM2.5) in NC/Nga mice. / Ogino, Keiki; Zhang, Ran; Takahashi, Hidekazu; Takemoto, Kei; Kubo, Masayuki; Murakami, Ikuo; Wang, Da Hong; Fujikura, Yoshihisa.

In: PLoS One, Vol. 9, No. 3, e92710, 26.03.2014.

Research output: Contribution to journalArticle

Ogino, K, Zhang, R, Takahashi, H, Takemoto, K, Kubo, M, Murakami, I, Wang, DH & Fujikura, Y 2014, 'Allergic airway inflammation by nasal inoculation of particulate matter (PM2.5) in NC/Nga mice', PLoS One, vol. 9, no. 3, e92710. https://doi.org/10.1371/journal.pone.0092710
Ogino K, Zhang R, Takahashi H, Takemoto K, Kubo M, Murakami I et al. Allergic airway inflammation by nasal inoculation of particulate matter (PM2.5) in NC/Nga mice. PLoS One. 2014 Mar 26;9(3). e92710. https://doi.org/10.1371/journal.pone.0092710
Ogino, Keiki ; Zhang, Ran ; Takahashi, Hidekazu ; Takemoto, Kei ; Kubo, Masayuki ; Murakami, Ikuo ; Wang, Da Hong ; Fujikura, Yoshihisa. / Allergic airway inflammation by nasal inoculation of particulate matter (PM2.5) in NC/Nga mice. In: PLoS One. 2014 ; Vol. 9, No. 3.
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