Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility

Christopher R. Clarke, Delphine Chinchilla, Sarah R. Hind, Fumiko Taguchi, Ryuji Miki, Yuki Ichinose, Gregory B. Martin, Scotland Leman, Georg Felix, Boris A. Vinatzer

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The bacterial flagellin (FliC) epitopes flg22 and flgII-28 are microbe-associated molecular patterns (MAMPs). Although flg22 is recognized by many plant species via the pattern recognition receptor FLS2, neither the flgII-28 receptor nor the extent of flgII-28 recognition by different plant families is known. Here, we tested the significance of flgII-28 as a MAMP and the importance of allelic diversity in flg22 and flgII-28 in plant-pathogen interactions using purified peptides and a Pseudomonas syringae {increment}fliC mutant complemented with different fliC alleles. The plant genotype and allelic diversity in flg22 and flgII-28 were found to significantly affect the plant immune response, but not bacterial motility. The recognition of flgII-28 is restricted to a number of solanaceous species. Although the flgII-28 peptide does not trigger any immune response in Arabidopsis, mutations in both flg22 and flgII-28 have FLS2-dependent effects on virulence. However, the expression of a tomato allele of FLS2 does not confer to Nicotiana benthamiana the ability to detect flgII-28, and tomato plants silenced for FLS2 are not altered in flgII-28 recognition. Therefore, MAMP diversification is an effective pathogen virulence strategy, and flgII-28 appears to be perceived by an as yet unidentified receptor in the Solanaceae, although it has an FLS2-dependent virulence effect in Arabidopsis.

Original languageEnglish
Pages (from-to)847-860
Number of pages14
JournalNew Phytologist
Volume200
Issue number3
DOIs
Publication statusPublished - Nov 2013

Fingerprint

bacterial motility
Plant Immunity
Pseudomonas syringae
Flagellin
flagellin
epitopes
Epitopes
immune response
Virulence
virulence
Lycopersicon esculentum
microorganisms
Arabidopsis
receptors
Alleles
tomatoes
peptides
Solanaceae
alleles
Pattern Recognition Receptors

Keywords

  • Flagellin
  • Flg22
  • FlgII-28
  • FLS2
  • Microbe-associated molecular pattern (MAMP)
  • Pathogen-associated molecular pattern (PAMP)
  • Pattern-triggered immunity (PTI)

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility. / Clarke, Christopher R.; Chinchilla, Delphine; Hind, Sarah R.; Taguchi, Fumiko; Miki, Ryuji; Ichinose, Yuki; Martin, Gregory B.; Leman, Scotland; Felix, Georg; Vinatzer, Boris A.

In: New Phytologist, Vol. 200, No. 3, 11.2013, p. 847-860.

Research output: Contribution to journalArticle

Clarke, CR, Chinchilla, D, Hind, SR, Taguchi, F, Miki, R, Ichinose, Y, Martin, GB, Leman, S, Felix, G & Vinatzer, BA 2013, 'Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility', New Phytologist, vol. 200, no. 3, pp. 847-860. https://doi.org/10.1111/nph.12408
Clarke, Christopher R. ; Chinchilla, Delphine ; Hind, Sarah R. ; Taguchi, Fumiko ; Miki, Ryuji ; Ichinose, Yuki ; Martin, Gregory B. ; Leman, Scotland ; Felix, Georg ; Vinatzer, Boris A. / Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility. In: New Phytologist. 2013 ; Vol. 200, No. 3. pp. 847-860.
@article{6076c3d35db74c5f9034eca156a44a54,
title = "Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility",
abstract = "The bacterial flagellin (FliC) epitopes flg22 and flgII-28 are microbe-associated molecular patterns (MAMPs). Although flg22 is recognized by many plant species via the pattern recognition receptor FLS2, neither the flgII-28 receptor nor the extent of flgII-28 recognition by different plant families is known. Here, we tested the significance of flgII-28 as a MAMP and the importance of allelic diversity in flg22 and flgII-28 in plant-pathogen interactions using purified peptides and a Pseudomonas syringae {increment}fliC mutant complemented with different fliC alleles. The plant genotype and allelic diversity in flg22 and flgII-28 were found to significantly affect the plant immune response, but not bacterial motility. The recognition of flgII-28 is restricted to a number of solanaceous species. Although the flgII-28 peptide does not trigger any immune response in Arabidopsis, mutations in both flg22 and flgII-28 have FLS2-dependent effects on virulence. However, the expression of a tomato allele of FLS2 does not confer to Nicotiana benthamiana the ability to detect flgII-28, and tomato plants silenced for FLS2 are not altered in flgII-28 recognition. Therefore, MAMP diversification is an effective pathogen virulence strategy, and flgII-28 appears to be perceived by an as yet unidentified receptor in the Solanaceae, although it has an FLS2-dependent virulence effect in Arabidopsis.",
keywords = "Flagellin, Flg22, FlgII-28, FLS2, Microbe-associated molecular pattern (MAMP), Pathogen-associated molecular pattern (PAMP), Pattern-triggered immunity (PTI)",
author = "Clarke, {Christopher R.} and Delphine Chinchilla and Hind, {Sarah R.} and Fumiko Taguchi and Ryuji Miki and Yuki Ichinose and Martin, {Gregory B.} and Scotland Leman and Georg Felix and Vinatzer, {Boris A.}",
year = "2013",
month = "11",
doi = "10.1111/nph.12408",
language = "English",
volume = "200",
pages = "847--860",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility

AU - Clarke, Christopher R.

AU - Chinchilla, Delphine

AU - Hind, Sarah R.

AU - Taguchi, Fumiko

AU - Miki, Ryuji

AU - Ichinose, Yuki

AU - Martin, Gregory B.

AU - Leman, Scotland

AU - Felix, Georg

AU - Vinatzer, Boris A.

PY - 2013/11

Y1 - 2013/11

N2 - The bacterial flagellin (FliC) epitopes flg22 and flgII-28 are microbe-associated molecular patterns (MAMPs). Although flg22 is recognized by many plant species via the pattern recognition receptor FLS2, neither the flgII-28 receptor nor the extent of flgII-28 recognition by different plant families is known. Here, we tested the significance of flgII-28 as a MAMP and the importance of allelic diversity in flg22 and flgII-28 in plant-pathogen interactions using purified peptides and a Pseudomonas syringae {increment}fliC mutant complemented with different fliC alleles. The plant genotype and allelic diversity in flg22 and flgII-28 were found to significantly affect the plant immune response, but not bacterial motility. The recognition of flgII-28 is restricted to a number of solanaceous species. Although the flgII-28 peptide does not trigger any immune response in Arabidopsis, mutations in both flg22 and flgII-28 have FLS2-dependent effects on virulence. However, the expression of a tomato allele of FLS2 does not confer to Nicotiana benthamiana the ability to detect flgII-28, and tomato plants silenced for FLS2 are not altered in flgII-28 recognition. Therefore, MAMP diversification is an effective pathogen virulence strategy, and flgII-28 appears to be perceived by an as yet unidentified receptor in the Solanaceae, although it has an FLS2-dependent virulence effect in Arabidopsis.

AB - The bacterial flagellin (FliC) epitopes flg22 and flgII-28 are microbe-associated molecular patterns (MAMPs). Although flg22 is recognized by many plant species via the pattern recognition receptor FLS2, neither the flgII-28 receptor nor the extent of flgII-28 recognition by different plant families is known. Here, we tested the significance of flgII-28 as a MAMP and the importance of allelic diversity in flg22 and flgII-28 in plant-pathogen interactions using purified peptides and a Pseudomonas syringae {increment}fliC mutant complemented with different fliC alleles. The plant genotype and allelic diversity in flg22 and flgII-28 were found to significantly affect the plant immune response, but not bacterial motility. The recognition of flgII-28 is restricted to a number of solanaceous species. Although the flgII-28 peptide does not trigger any immune response in Arabidopsis, mutations in both flg22 and flgII-28 have FLS2-dependent effects on virulence. However, the expression of a tomato allele of FLS2 does not confer to Nicotiana benthamiana the ability to detect flgII-28, and tomato plants silenced for FLS2 are not altered in flgII-28 recognition. Therefore, MAMP diversification is an effective pathogen virulence strategy, and flgII-28 appears to be perceived by an as yet unidentified receptor in the Solanaceae, although it has an FLS2-dependent virulence effect in Arabidopsis.

KW - Flagellin

KW - Flg22

KW - FlgII-28

KW - FLS2

KW - Microbe-associated molecular pattern (MAMP)

KW - Pathogen-associated molecular pattern (PAMP)

KW - Pattern-triggered immunity (PTI)

UR - http://www.scopus.com/inward/record.url?scp=84885431170&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885431170&partnerID=8YFLogxK

U2 - 10.1111/nph.12408

DO - 10.1111/nph.12408

M3 - Article

C2 - 23865782

AN - SCOPUS:84885431170

VL - 200

SP - 847

EP - 860

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 3

ER -