TY - JOUR
T1 - Allelic loss of the ING gene family loci is a frequent event in ameloblastoma
AU - Borkosky, Silvia S.
AU - Gunduz, Mehmet
AU - Beder, Levent
AU - Tsujigiwa, Hidetsugu
AU - Tamamura, Ryo
AU - Gunduz, Esra
AU - Katase, Naoki
AU - Rodriguez, Andrea P.
AU - Sasaki, Akira
AU - Nagai, Noriyuki
AU - Nagatsuka, Hitoshi
PY - 2010
Y1 - 2010
N2 - Ameloblastoma is the most frequently encountered odontogenic tumor, characterized by a locally invasive behavior, frequent recurrences, and, although rare, metastatic capacity. Loss or inactivation of tumor suppressor genes (TSGs) allows cells to acquire neoplastic growth. The ING family proteins are tumor suppressors that physically and functionally interact with p53 to perform important roles in apoptosis, DNA repair, cell cycle regulation, and senescence. TP53 genetic alterations were reported to infrequently occur in ameloblastoma. Considering that other TSGs related to TP53 could be altered in this tumor, we focused our study on the ING family genes. We analyzed the loss of heterozygosity (LOH) status of the ING family (ING1-ING5) chromosomal loci in a group of ameloblastomas by microsatellite analysis, and correlated the ING LOH status with clinicopathological characteristics. By using specific microsatellite markers, high frequency of LOH was found at the loci of each ING gene family member (33.3-72.2%). A significant relationship was shown between LOH of D2S140 (ING5 locus) and solid tumor type (p = 0.02). LOH of ING3MS (ING3 locus) was also high in solid type tumors, showing a near significant association. In addition, a notable tendency toward higher LOH for half of the markers was observed in recurrent cases. LOH of ING family genes appears as a common genetic alteration in solid ameloblastoma. The current study provides interesting novel information regarding the potential prognostic significance of the allelic loss of the ING gene family loci in ameloblastoma tumorigenesis.
AB - Ameloblastoma is the most frequently encountered odontogenic tumor, characterized by a locally invasive behavior, frequent recurrences, and, although rare, metastatic capacity. Loss or inactivation of tumor suppressor genes (TSGs) allows cells to acquire neoplastic growth. The ING family proteins are tumor suppressors that physically and functionally interact with p53 to perform important roles in apoptosis, DNA repair, cell cycle regulation, and senescence. TP53 genetic alterations were reported to infrequently occur in ameloblastoma. Considering that other TSGs related to TP53 could be altered in this tumor, we focused our study on the ING family genes. We analyzed the loss of heterozygosity (LOH) status of the ING family (ING1-ING5) chromosomal loci in a group of ameloblastomas by microsatellite analysis, and correlated the ING LOH status with clinicopathological characteristics. By using specific microsatellite markers, high frequency of LOH was found at the loci of each ING gene family member (33.3-72.2%). A significant relationship was shown between LOH of D2S140 (ING5 locus) and solid tumor type (p = 0.02). LOH of ING3MS (ING3 locus) was also high in solid type tumors, showing a near significant association. In addition, a notable tendency toward higher LOH for half of the markers was observed in recurrent cases. LOH of ING family genes appears as a common genetic alteration in solid ameloblastoma. The current study provides interesting novel information regarding the potential prognostic significance of the allelic loss of the ING gene family loci in ameloblastoma tumorigenesis.
KW - Allelic loss
KW - Ameloblastoma
KW - ING family
KW - Microsatellite
KW - Tumor suppressor genes
UR - http://www.scopus.com/inward/record.url?scp=77954694838&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954694838&partnerID=8YFLogxK
U2 - 10.3727/096504010X12704916124864
DO - 10.3727/096504010X12704916124864
M3 - Article
C2 - 20681410
AN - SCOPUS:77954694838
VL - 18
SP - 509
EP - 518
JO - Cancer Communications
JF - Cancer Communications
SN - 0965-0407
IS - 10
ER -
