Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers.

Hiromi Sasamoto, Takeshi Nagasaka, Kenji Notohara, Kazuhide Ozaki, Hiroshi Isozaki, Noriaki Tanaka, Nagahide Matsubara

Research output: Contribution to journalArticle

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Abstract

H19 and IGF2 genes are imprinted genes and expressed differently depending on whether they are carried by a chromosome of maternal or paternal origin; H19 is expressed only from the maternal allele and IGF2 only from the paternally inherited allele. The upstream promoter region of H19 has the imprinting-control region (ICR) or CTCF binding sites, where the methylation status of this region is critical to the regulation of imprinting of the H19/IGF2 locus located in chromosome 11p15. There are various reports on imprinting disorders in this region. In colorectal cancer aberrant biallelic methylation of CTCF binding site has been reported, and aberrant hypomethylation of this region in bladder cancer. Thus, certain human neoplasms have either hyper- or hypo-methylation in the ICR. Hence it is still difficult to analyze allele-specific methylation disorder of the region, or differentially methylated regions (DMR), locate upstream of H19. Here we report a new method, which could distinguish paternal epigenetic or maternal epigenetic pattern by a single PCR assay, to combine methylation-specific PCR and PCR with confronting two-pair primers (MSP-CTPP). Using this method, we investigated the region close to H19 ICR in 161 colorectal cancer and 65 gastric cancer cases.

Original languageEnglish
Pages (from-to)1273-1278
Number of pages6
JournalInternational Journal of Oncology
Volume25
Issue number5
Publication statusPublished - Nov 2004

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Genetic Promoter Regions
Methylation
Alleles
Polymerase Chain Reaction
Mothers
Epigenomics
Stomach Neoplasms
Colorectal Neoplasms
Chromosomes
Binding Sites
Urinary Bladder Neoplasms
Genes
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sasamoto, H., Nagasaka, T., Notohara, K., Ozaki, K., Isozaki, H., Tanaka, N., & Matsubara, N. (2004). Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers. International Journal of Oncology, 25(5), 1273-1278.

Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers. / Sasamoto, Hiromi; Nagasaka, Takeshi; Notohara, Kenji; Ozaki, Kazuhide; Isozaki, Hiroshi; Tanaka, Noriaki; Matsubara, Nagahide.

In: International Journal of Oncology, Vol. 25, No. 5, 11.2004, p. 1273-1278.

Research output: Contribution to journalArticle

Sasamoto, H, Nagasaka, T, Notohara, K, Ozaki, K, Isozaki, H, Tanaka, N & Matsubara, N 2004, 'Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers.', International Journal of Oncology, vol. 25, no. 5, pp. 1273-1278.
Sasamoto, Hiromi ; Nagasaka, Takeshi ; Notohara, Kenji ; Ozaki, Kazuhide ; Isozaki, Hiroshi ; Tanaka, Noriaki ; Matsubara, Nagahide. / Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers. In: International Journal of Oncology. 2004 ; Vol. 25, No. 5. pp. 1273-1278.
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