All four members of the Ten-m/Odz family of transmembrane proteins form dimers

Kang Feng, Xiao Hong Zhou, Toshitaka Oohashi, Matthias Mörgelin, Ariel Lustig, Satoshi Hirakawa, Yoshifumi Ninomiya, Jürgen Engel, Uwe Rauch, Reinhard Fässler

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Abstract

Ten-m/Odz/teneurins are a new family of four distinct type II transmembrane molecules. Their extracellular domains are composed of an array of eight consecutive EGF modules followed by a large globular domain. Two of the eight modules contain only 5 instead of the typical 6 cysteine residues and have the capability to dimerize in a covalent, disulfide-linked fashion. The structural properties of the extracellular domains of all four mouse Ten-m proteins have been analyzed using secreted, recombinant molecules produced by mammalian HEK-293 cells. Electron microscopic analysis supported by analytical ultracentrifugation data revealed that the recombinant extracellular domains of all Ten-m proteins formed homodimers. SDS-PAGE analysis under nonreducing conditions as well as negative staining after partial denaturation of the molecules indicated that the globular COOH-terminal domains of Ten-m1 and -m4 contained subdomains with a pronounced stability against denaturing agents, especially when compared with the homologous domains of Ten-m2 and -m3. Co-transfection experiments of mammalian cells with two different extracellular domains revealed that Ten-m molecules have also the ability to form heterodimers, a property that, combined with alternative splicing events, allows the formation of a multitude of molecules with different characteristics from a limited set of genes.

Original languageEnglish
Pages (from-to)26128-26135
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number29
DOIs
Publication statusPublished - Jul 19 2002

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Feng, K., Zhou, X. H., Oohashi, T., Mörgelin, M., Lustig, A., Hirakawa, S., Ninomiya, Y., Engel, J., Rauch, U., & Fässler, R. (2002). All four members of the Ten-m/Odz family of transmembrane proteins form dimers. Journal of Biological Chemistry, 277(29), 26128-26135. https://doi.org/10.1074/jbc.M203722200