Alginate/aminopropyl-silicate/alginate membrane immunoisolatability and insulin secretion of encapsulated islets

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We utilized the sol-gel reaction to prepare an immunoisolatable membrane for a microcapsule-shaped bioartificial pancreas. The membrane, derived from two precursors, 3-(aminopropyl)trimethoxysilane (APTrMOS) and tetramethoxysilane (TMOS), was formed onto calcium-alginate gel beads via electrostatic interaction. The molecular weight cutoff point of less than 150 000 required for immunoisolation was achieved at molar ratios ([APTrMOS]/[TMOS]) ranging from 0.60 to 2.40 with the amount of APTrMOS fixed at 3.40 mmol/(10 mL of calcium-alginate). When encapsulated in a membrane prepared at the molar ratio of 0.60, the islets contracted in volume and showed no response to stimulation by a high glucose concentration. However, islets in a membrane prepared at the molar ratio of 2.40 showed no contraction and responded to the glucose stimulation at almost the same level as free islets. These results demonstrated that the molar ratio of the precursors was a dominant factor affecting membrane permeability and the insulin secretion activity of the encapsulated islets.

Original languageEnglish
Pages (from-to)401-403
Number of pages3
JournalBiotechnology Progress
Volume18
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

calcium alginate
Silicates
insulin secretion
alginates
silicates
Insulin
alginate gels
glucose
Membranes
electrostatic interactions
membrane permeability
pancreas
gels
molecular weight
Gels
Glucose
Polymethyl Methacrylate
Static Electricity
Capsules
Pancreas

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

Cite this

Alginate/aminopropyl-silicate/alginate membrane immunoisolatability and insulin secretion of encapsulated islets. / Sakai, Shinji; Ono, Tsutomu; Ijima, Hiroyuki; Kawakami, Koei.

In: Biotechnology Progress, Vol. 18, No. 2, 2002, p. 401-403.

Research output: Contribution to journalArticle

@article{efed37ae937149f19c39b37e3c826402,
title = "Alginate/aminopropyl-silicate/alginate membrane immunoisolatability and insulin secretion of encapsulated islets",
abstract = "We utilized the sol-gel reaction to prepare an immunoisolatable membrane for a microcapsule-shaped bioartificial pancreas. The membrane, derived from two precursors, 3-(aminopropyl)trimethoxysilane (APTrMOS) and tetramethoxysilane (TMOS), was formed onto calcium-alginate gel beads via electrostatic interaction. The molecular weight cutoff point of less than 150 000 required for immunoisolation was achieved at molar ratios ([APTrMOS]/[TMOS]) ranging from 0.60 to 2.40 with the amount of APTrMOS fixed at 3.40 mmol/(10 mL of calcium-alginate). When encapsulated in a membrane prepared at the molar ratio of 0.60, the islets contracted in volume and showed no response to stimulation by a high glucose concentration. However, islets in a membrane prepared at the molar ratio of 2.40 showed no contraction and responded to the glucose stimulation at almost the same level as free islets. These results demonstrated that the molar ratio of the precursors was a dominant factor affecting membrane permeability and the insulin secretion activity of the encapsulated islets.",
author = "Shinji Sakai and Tsutomu Ono and Hiroyuki Ijima and Koei Kawakami",
year = "2002",
doi = "10.1021/bp010147+",
language = "English",
volume = "18",
pages = "401--403",
journal = "Biotechnology Progress",
issn = "8756-7938",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

TY - JOUR

T1 - Alginate/aminopropyl-silicate/alginate membrane immunoisolatability and insulin secretion of encapsulated islets

AU - Sakai, Shinji

AU - Ono, Tsutomu

AU - Ijima, Hiroyuki

AU - Kawakami, Koei

PY - 2002

Y1 - 2002

N2 - We utilized the sol-gel reaction to prepare an immunoisolatable membrane for a microcapsule-shaped bioartificial pancreas. The membrane, derived from two precursors, 3-(aminopropyl)trimethoxysilane (APTrMOS) and tetramethoxysilane (TMOS), was formed onto calcium-alginate gel beads via electrostatic interaction. The molecular weight cutoff point of less than 150 000 required for immunoisolation was achieved at molar ratios ([APTrMOS]/[TMOS]) ranging from 0.60 to 2.40 with the amount of APTrMOS fixed at 3.40 mmol/(10 mL of calcium-alginate). When encapsulated in a membrane prepared at the molar ratio of 0.60, the islets contracted in volume and showed no response to stimulation by a high glucose concentration. However, islets in a membrane prepared at the molar ratio of 2.40 showed no contraction and responded to the glucose stimulation at almost the same level as free islets. These results demonstrated that the molar ratio of the precursors was a dominant factor affecting membrane permeability and the insulin secretion activity of the encapsulated islets.

AB - We utilized the sol-gel reaction to prepare an immunoisolatable membrane for a microcapsule-shaped bioartificial pancreas. The membrane, derived from two precursors, 3-(aminopropyl)trimethoxysilane (APTrMOS) and tetramethoxysilane (TMOS), was formed onto calcium-alginate gel beads via electrostatic interaction. The molecular weight cutoff point of less than 150 000 required for immunoisolation was achieved at molar ratios ([APTrMOS]/[TMOS]) ranging from 0.60 to 2.40 with the amount of APTrMOS fixed at 3.40 mmol/(10 mL of calcium-alginate). When encapsulated in a membrane prepared at the molar ratio of 0.60, the islets contracted in volume and showed no response to stimulation by a high glucose concentration. However, islets in a membrane prepared at the molar ratio of 2.40 showed no contraction and responded to the glucose stimulation at almost the same level as free islets. These results demonstrated that the molar ratio of the precursors was a dominant factor affecting membrane permeability and the insulin secretion activity of the encapsulated islets.

UR - http://www.scopus.com/inward/record.url?scp=0036010298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036010298&partnerID=8YFLogxK

U2 - 10.1021/bp010147+

DO - 10.1021/bp010147+

M3 - Article

C2 - 11934313

AN - SCOPUS:0036010298

VL - 18

SP - 401

EP - 403

JO - Biotechnology Progress

JF - Biotechnology Progress

SN - 8756-7938

IS - 2

ER -