Albumin stimulates monocyte chemotactic protein-1 expression in rat embryonic mixed brain cells

C. F. Calvo, Edwige Amigou, Martine Tencé, Teizo Yoshimura, Jacques Glowinski

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Albumin, a blood protein absent from the adult brain in physiological situations, can be brought into contact with brain cells during development or, in adult, following breakdown of the blood-brain barrier occurring as a result of local inflammation. In the present study, we show that ovalbumin and albumin induce the release of monocyte chemotactic protein 1 (MCP-1/CCL2) from rat embryonic mixed brain cells. A short-term exposure to ovalbumin during the cell dissociation procedure is sufficient to generate MCP-1 mRNA. A comparable effect is observed when the cells are incubated for 4 hr with ovalbumin or rat albumin, while MCP-1 messengers are barely detectable following bovine albumin exposure. The amount of MCP-1 protein measured in 4 hr-supernatants of albumin-treated cells followed the same albumin-inducing pattern as that of MCP-1 mRNA, while all albumins tested induced MCP-1 protein after a 17 hr-incubation period. The albumin-induced MCP-1 production is significantly inhibited in calphostin C-treated cells, suggesting the implication of a protein kinase C-dependent signaling pathway. This MCP-1-inducing activity is maintained after a lipid extraction procedure but abolished by proteinase K or trypsin treatments of albumin. The MCP-1 secretion following albumin contact with nervous cells could thus interfere, by chemotactic gradient formation, with the brain infiltration program of blood-derived cells during development or brain injury.

Original languageEnglish
Pages (from-to)707-714
Number of pages8
JournalJournal of Neuroscience Research
Volume80
Issue number5
DOIs
Publication statusPublished - Jun 1 2005
Externally publishedYes

Fingerprint

Chemokine CCL2
Albumins
Brain
Ovalbumin
Endopeptidase K
Messenger RNA
Blood-Brain Barrier
Trypsin
Brain Injuries
Protein Kinase C
Blood Proteins
Blood Cells
Proteins
Inflammation
Lipids

Keywords

  • Agonist
  • Albumins
  • Brain
  • Cytology
  • Monocyte chemoattractant protein-1
  • Pharmacology

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Albumin stimulates monocyte chemotactic protein-1 expression in rat embryonic mixed brain cells. / Calvo, C. F.; Amigou, Edwige; Tencé, Martine; Yoshimura, Teizo; Glowinski, Jacques.

In: Journal of Neuroscience Research, Vol. 80, No. 5, 01.06.2005, p. 707-714.

Research output: Contribution to journalArticle

Calvo, C. F. ; Amigou, Edwige ; Tencé, Martine ; Yoshimura, Teizo ; Glowinski, Jacques. / Albumin stimulates monocyte chemotactic protein-1 expression in rat embryonic mixed brain cells. In: Journal of Neuroscience Research. 2005 ; Vol. 80, No. 5. pp. 707-714.
@article{17ba687237134d098341e3df9aa4da1f,
title = "Albumin stimulates monocyte chemotactic protein-1 expression in rat embryonic mixed brain cells",
abstract = "Albumin, a blood protein absent from the adult brain in physiological situations, can be brought into contact with brain cells during development or, in adult, following breakdown of the blood-brain barrier occurring as a result of local inflammation. In the present study, we show that ovalbumin and albumin induce the release of monocyte chemotactic protein 1 (MCP-1/CCL2) from rat embryonic mixed brain cells. A short-term exposure to ovalbumin during the cell dissociation procedure is sufficient to generate MCP-1 mRNA. A comparable effect is observed when the cells are incubated for 4 hr with ovalbumin or rat albumin, while MCP-1 messengers are barely detectable following bovine albumin exposure. The amount of MCP-1 protein measured in 4 hr-supernatants of albumin-treated cells followed the same albumin-inducing pattern as that of MCP-1 mRNA, while all albumins tested induced MCP-1 protein after a 17 hr-incubation period. The albumin-induced MCP-1 production is significantly inhibited in calphostin C-treated cells, suggesting the implication of a protein kinase C-dependent signaling pathway. This MCP-1-inducing activity is maintained after a lipid extraction procedure but abolished by proteinase K or trypsin treatments of albumin. The MCP-1 secretion following albumin contact with nervous cells could thus interfere, by chemotactic gradient formation, with the brain infiltration program of blood-derived cells during development or brain injury.",
keywords = "Agonist, Albumins, Brain, Cytology, Monocyte chemoattractant protein-1, Pharmacology",
author = "Calvo, {C. F.} and Edwige Amigou and Martine Tenc{\'e} and Teizo Yoshimura and Jacques Glowinski",
year = "2005",
month = "6",
day = "1",
doi = "10.1002/jnr.20511",
language = "English",
volume = "80",
pages = "707--714",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "5",

}

TY - JOUR

T1 - Albumin stimulates monocyte chemotactic protein-1 expression in rat embryonic mixed brain cells

AU - Calvo, C. F.

AU - Amigou, Edwige

AU - Tencé, Martine

AU - Yoshimura, Teizo

AU - Glowinski, Jacques

PY - 2005/6/1

Y1 - 2005/6/1

N2 - Albumin, a blood protein absent from the adult brain in physiological situations, can be brought into contact with brain cells during development or, in adult, following breakdown of the blood-brain barrier occurring as a result of local inflammation. In the present study, we show that ovalbumin and albumin induce the release of monocyte chemotactic protein 1 (MCP-1/CCL2) from rat embryonic mixed brain cells. A short-term exposure to ovalbumin during the cell dissociation procedure is sufficient to generate MCP-1 mRNA. A comparable effect is observed when the cells are incubated for 4 hr with ovalbumin or rat albumin, while MCP-1 messengers are barely detectable following bovine albumin exposure. The amount of MCP-1 protein measured in 4 hr-supernatants of albumin-treated cells followed the same albumin-inducing pattern as that of MCP-1 mRNA, while all albumins tested induced MCP-1 protein after a 17 hr-incubation period. The albumin-induced MCP-1 production is significantly inhibited in calphostin C-treated cells, suggesting the implication of a protein kinase C-dependent signaling pathway. This MCP-1-inducing activity is maintained after a lipid extraction procedure but abolished by proteinase K or trypsin treatments of albumin. The MCP-1 secretion following albumin contact with nervous cells could thus interfere, by chemotactic gradient formation, with the brain infiltration program of blood-derived cells during development or brain injury.

AB - Albumin, a blood protein absent from the adult brain in physiological situations, can be brought into contact with brain cells during development or, in adult, following breakdown of the blood-brain barrier occurring as a result of local inflammation. In the present study, we show that ovalbumin and albumin induce the release of monocyte chemotactic protein 1 (MCP-1/CCL2) from rat embryonic mixed brain cells. A short-term exposure to ovalbumin during the cell dissociation procedure is sufficient to generate MCP-1 mRNA. A comparable effect is observed when the cells are incubated for 4 hr with ovalbumin or rat albumin, while MCP-1 messengers are barely detectable following bovine albumin exposure. The amount of MCP-1 protein measured in 4 hr-supernatants of albumin-treated cells followed the same albumin-inducing pattern as that of MCP-1 mRNA, while all albumins tested induced MCP-1 protein after a 17 hr-incubation period. The albumin-induced MCP-1 production is significantly inhibited in calphostin C-treated cells, suggesting the implication of a protein kinase C-dependent signaling pathway. This MCP-1-inducing activity is maintained after a lipid extraction procedure but abolished by proteinase K or trypsin treatments of albumin. The MCP-1 secretion following albumin contact with nervous cells could thus interfere, by chemotactic gradient formation, with the brain infiltration program of blood-derived cells during development or brain injury.

KW - Agonist

KW - Albumins

KW - Brain

KW - Cytology

KW - Monocyte chemoattractant protein-1

KW - Pharmacology

UR - http://www.scopus.com/inward/record.url?scp=21244447452&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21244447452&partnerID=8YFLogxK

U2 - 10.1002/jnr.20511

DO - 10.1002/jnr.20511

M3 - Article

VL - 80

SP - 707

EP - 714

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 5

ER -