Al-induced secretion of organic acid, gene expression and root elongation in soybean roots

Daoming Wu, Man Zhao, Shouying Shen, Youqiang Fu, Takayuki Sasaki, Yoko Yamamoto, Wenhui Wei, Hong Shen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Al-activated organic acid transporter genes (ALMT and MATE) and plasma membrane H+-ATPase gene (PHA) are known to contribute to the regulation of organic acid secretion in several crops. However, it remains unclear how these genes interact to modulate organic acid exudation in the same plant species. In this study, Al-induced expression of genes (GmALMT1, GmMATE1 and GmPHA1), secretion of organic acid and root elongation were characterized in soybean roots. Results indicated that treatment with 50 μM Al activated the expression of GmALMT1, GmMATE1 and GmPHA1, and the exudation of citrate and malate significantly in apical 5 mm region of soybean seedlings, but inhibited root elongation by 57. 8 %. The highest malate exudation rate and the maximal expression of GmALMT1 and GmPHA1 were observed after 2 h of 50 μM Al treatment, while the corresponding values for citrate exudation rate and GmMATE1 expression occurred at 8 h. The exudation of malate and citrate contributed to but could not recover Al-triggered root elongation. A root-split experiment indicated that Al-activated gene expression, organic acid secretion and root growth inhibition required the direct contact of Al3+. The removal of shoots in soybean seedlings decreased Al-activated gene expression by 26. 1-40. 5 %, and secretion of organic acid by 14. 7-40. 2 %. Furthermore, shoot excision aggravated Al-inhibited root elongation, indicating the existence of other Al tolerance mechanism except the exudation of organic acid. These results suggested that Al-activated expression of GmPHA1-, GmMATE1- and GmALMT1-mediated exudation of malate and citrate, and shoots played an important role in Al toxicity resistance in soybean roots.

Original languageEnglish
Pages (from-to)223-232
Number of pages10
JournalActa Physiologiae Plantarum
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

exudation
Soybeans
organic acids and salts
root growth
secretion
soybeans
Gene Expression
gene expression
malates
Acids
Citric Acid
citrates
Seedlings
shoots
Genes
H-transporting ATP synthase
Proton-Translocating ATPases
genes
seedlings
direct contact

Keywords

  • Aluminium
  • GmALMT1
  • GmMATE1
  • GmPHA1
  • Organic acid

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Al-induced secretion of organic acid, gene expression and root elongation in soybean roots. / Wu, Daoming; Zhao, Man; Shen, Shouying; Fu, Youqiang; Sasaki, Takayuki; Yamamoto, Yoko; Wei, Wenhui; Shen, Hong.

In: Acta Physiologiae Plantarum, Vol. 35, No. 1, 01.2013, p. 223-232.

Research output: Contribution to journalArticle

Wu, Daoming ; Zhao, Man ; Shen, Shouying ; Fu, Youqiang ; Sasaki, Takayuki ; Yamamoto, Yoko ; Wei, Wenhui ; Shen, Hong. / Al-induced secretion of organic acid, gene expression and root elongation in soybean roots. In: Acta Physiologiae Plantarum. 2013 ; Vol. 35, No. 1. pp. 223-232.
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