Agrobacterium tumefaciens-mediated transformation as a tool for random mutagenesis of Colletotrichum trifolii

Hiroyuki Takahara, Gento Tsuji, Yasuyuki Kubo, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

We transformed Colletotrichum trifolii, the causal agent of alfalfa anthracnose, using Agrobacterium tumefaciens as a new tool for random insertional mutagenesis. Fungal spores of C. trifolii were transformed with T-DNA including the hygromycin phosphotransferase gene (hph). Southern analysis showed that every randomly selected transformant had a unique hybridization pattern of T-DNA, suggesting that the T-DNA was randomly integrated into the fungal genome. More significantly, about 75% of transformants had a single copy of the T-DNA. The results demonstrate that insertional mutagenesis via A. tumefaciens is a useful tool for studying the function of C. trifolii genes.

Original languageEnglish
Pages (from-to)93-96
Number of pages4
JournalJournal of General Plant Pathology
Volume70
Issue number2
DOIs
Publication statusPublished - Apr 2004

Keywords

  • Agrobacterium tumefaciens
  • Anthracnose
  • Colletotrichum trifolii
  • Random insertional mutagenesis
  • T-DNA
  • Transformation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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