Agrobacterium tumefaciens Enhances Biosynthesis of Two Distinct Auxins in the Formation of Crown Galls

Kiyoshi Mashiguchi, Hiroshi Hisano, Noriko Takeda-Kamiya, Yumiko Takebayashi, Tohru Ariizumi, Yangbin Gao, Hiroshi Ezura, Kazuhiro Sato, Yunde Zhao, Ken Ichiro Hayashi, Hiroyuki Kasahara

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The plant pathogen Agrobacterium tumefaciens infects plants and introduces the transferred-DNA (T-DNA) region of the Ti-plasmid into nuclear DNA of host plants to induce the formation of tumors (crown galls). The T-DNA region carries iaaM and iaaH genes for synthesis of the plant hormone auxin, indole-3-acetic acid (IAA). It has been demonstrated that the iaaM gene encodes a tryptophan 2-monooxygenase which catalyzes the conversion of tryptophan to indole-3-acetamide (IAM), and the iaaH gene encodes an amidase for subsequent conversion of IAM to IAA. In this article, we demonstrate that A. tumefaciens enhances the production of both IAA and phenylacetic acid (PAA), another auxin which does not show polar transport characteristics, in the formation of crown galls. Using liquid chromatography-tandem mass spectroscopy, we found that the endogenous levels of phenylacetamide (PAM) and PAA metabolites, as well as IAM and IAA metabolites, are remarkably increased in crown galls formed on the stem of tomato plants, implying that two distinct auxins are simultaneously synthesized via the IaaM-IaaH pathway. Moreover, we found that the induction of the iaaM gene dramatically elevated the levels of PAM, PAA and its metabolites, along with IAM, IAA and its metabolites, in Arabidopsis and barley. From these results, we conclude that A. tumefaciens enhances biosynthesis of two distinct auxins in the formation of crown galls.

Original languageEnglish
Pages (from-to)29-37
Number of pages9
JournalPlant & cell physiology
Volume60
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Plant Tumors
crown galls
Agrobacterium tumefaciens
Indoleacetic Acids
acetamides
Agrobacterium radiobacter
indole acetic acid
auxins
indoles
phenylacetic acid
biosynthesis
metabolites
amidase
tryptophan
Genes
Plant Tumor-Inducing Plasmids
Plant DNA
Plant Stems
Plant Growth Regulators
genes

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Mashiguchi, K., Hisano, H., Takeda-Kamiya, N., Takebayashi, Y., Ariizumi, T., Gao, Y., ... Kasahara, H. (2019). Agrobacterium tumefaciens Enhances Biosynthesis of Two Distinct Auxins in the Formation of Crown Galls. Plant & cell physiology, 60(1), 29-37. https://doi.org/10.1093/pcp/pcy182

Agrobacterium tumefaciens Enhances Biosynthesis of Two Distinct Auxins in the Formation of Crown Galls. / Mashiguchi, Kiyoshi; Hisano, Hiroshi; Takeda-Kamiya, Noriko; Takebayashi, Yumiko; Ariizumi, Tohru; Gao, Yangbin; Ezura, Hiroshi; Sato, Kazuhiro; Zhao, Yunde; Hayashi, Ken Ichiro; Kasahara, Hiroyuki.

In: Plant & cell physiology, Vol. 60, No. 1, 01.01.2019, p. 29-37.

Research output: Contribution to journalArticle

Mashiguchi, K, Hisano, H, Takeda-Kamiya, N, Takebayashi, Y, Ariizumi, T, Gao, Y, Ezura, H, Sato, K, Zhao, Y, Hayashi, KI & Kasahara, H 2019, 'Agrobacterium tumefaciens Enhances Biosynthesis of Two Distinct Auxins in the Formation of Crown Galls', Plant & cell physiology, vol. 60, no. 1, pp. 29-37. https://doi.org/10.1093/pcp/pcy182
Mashiguchi, Kiyoshi ; Hisano, Hiroshi ; Takeda-Kamiya, Noriko ; Takebayashi, Yumiko ; Ariizumi, Tohru ; Gao, Yangbin ; Ezura, Hiroshi ; Sato, Kazuhiro ; Zhao, Yunde ; Hayashi, Ken Ichiro ; Kasahara, Hiroyuki. / Agrobacterium tumefaciens Enhances Biosynthesis of Two Distinct Auxins in the Formation of Crown Galls. In: Plant & cell physiology. 2019 ; Vol. 60, No. 1. pp. 29-37.
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