Agrobacterium tumefaciens AK-6b gene modulates phenolic compound metabolism in tobacco

Ivan Galis, Yasutaka Kakiuchi, Petr Šimek, Hiroetsu Wabiko

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The 6b gene (AK-6b) of Agrobacterium tumefaciens AKE10 can substitute for the requirement of tobacco tissues for auxin and cytokinin to maintain callus growth in the culture medium. To identify compounds that might be involved in this process we analyzed phenolic metabolites in transgenic tobacco tissues expressing the AK-6b gene. On medium containing both cytokinin and auxin (SH medium), transgenic calli accumulated higher levels of chlorogenic acid, caffeoyl putrescine, rutin and kaempferol-3-rutinoside, than did wild-type tissues. In contrast, the levels of scopolin and its aglycone, scopoletin were lower in transgenic tissues. On hormone-free medium, these phenolic compounds showed neither significant levels nor an apparent relationship with AK-6b transcript levels, except for the negatively correlated levels of scopoletin and AK-6b transcripts. Apparently, the AK-6b gene acts, in SH medium, to redirect the synthesis of scopolin in tobacco tissues towards the preferential synthesis of caffeic acid derivatives and flavonoids.

Original languageEnglish
Pages (from-to)169-179
Number of pages11
JournalPhytochemistry
Volume65
Issue number2
DOIs
Publication statusPublished - Jan 2004
Externally publishedYes

Fingerprint

Agrobacterium tumefaciens
Tobacco
Agrobacterium radiobacter
Metabolism
phenolic compounds
tobacco
Genes
Tissue
metabolism
Scopoletin
scopoletin
Cytokinins
Indoleacetic Acids
Bony Callus
genetically modified organisms
rutin
genes
cytokinins
auxins
callus

Keywords

  • Caffeoylputrescine
  • Chlorogenic acid
  • Flavonoids
  • Kaempferol-3-rutinoside
  • Metabolism
  • Nicotiana tabacum
  • Phenylpropanoids
  • Rutin
  • Scopoletin
  • Scopolin
  • Solanaceae
  • Tobacco

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery

Cite this

Agrobacterium tumefaciens AK-6b gene modulates phenolic compound metabolism in tobacco. / Galis, Ivan; Kakiuchi, Yasutaka; Šimek, Petr; Wabiko, Hiroetsu.

In: Phytochemistry, Vol. 65, No. 2, 01.2004, p. 169-179.

Research output: Contribution to journalArticle

Galis, Ivan ; Kakiuchi, Yasutaka ; Šimek, Petr ; Wabiko, Hiroetsu. / Agrobacterium tumefaciens AK-6b gene modulates phenolic compound metabolism in tobacco. In: Phytochemistry. 2004 ; Vol. 65, No. 2. pp. 169-179.
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abstract = "The 6b gene (AK-6b) of Agrobacterium tumefaciens AKE10 can substitute for the requirement of tobacco tissues for auxin and cytokinin to maintain callus growth in the culture medium. To identify compounds that might be involved in this process we analyzed phenolic metabolites in transgenic tobacco tissues expressing the AK-6b gene. On medium containing both cytokinin and auxin (SH medium), transgenic calli accumulated higher levels of chlorogenic acid, caffeoyl putrescine, rutin and kaempferol-3-rutinoside, than did wild-type tissues. In contrast, the levels of scopolin and its aglycone, scopoletin were lower in transgenic tissues. On hormone-free medium, these phenolic compounds showed neither significant levels nor an apparent relationship with AK-6b transcript levels, except for the negatively correlated levels of scopoletin and AK-6b transcripts. Apparently, the AK-6b gene acts, in SH medium, to redirect the synthesis of scopolin in tobacco tissues towards the preferential synthesis of caffeic acid derivatives and flavonoids.",
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