Agrobacterium-mediated transformation of switchgrass and inheritance of the transgenes

Yajun Xi, Chunxiang Fu, Yaxin Ge, Rangaraj Nandakumar, Hiroshi Hisano, Joseph Bouton, Zeng Yu Wang

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Switchgrass (Panicum virgatum L.) has been developed into an important biofuel crop. Embryogenic calli induced from caryopses or inflorescences of the lowland switchgrass cultivar Alamo were used for Agrobacterium-mediated transformation. A chimeric hygromycin phosphotransferase gene (hph) was used as the selectable marker and hygromycin as the selection agent. Embryogenic calli were infected with Agrobacterium tumefaciens strain EHA105. Calli resistant to hygromycin were obtained after 5 to 8 weeks of selection. Soil-grown transgenic switchgrass plants were obtained 4 to 5 months after Agrobacterium infection. The transgenic nature of the regenerated plants was demonstrated by PCR, Southern blot hybridization analysis, and GUS staining. T1 progeny were obtained after reciprocal crosses between transgenic and untransformed control plants. Molecular analyses of the T1 progeny revealed various patterns of segregation. Transgene silencing was observed in the progeny with multiple inserts. Interestingly, reversal of the expression of the silenced transgene was found in segregating progeny with a single insert.

Original languageEnglish
Pages (from-to)275-283
Number of pages9
JournalBioenergy Research
Volume2
Issue number4
DOIs
Publication statusPublished - Nov 2009
Externally publishedYes

Fingerprint

Panicum virgatum
Biofuels
Agrobacterium
transgenes
Crops
inheritance (genetics)
Genes
Soils
callus
genetically modified organisms
energy crops
reciprocal crosses
Agrobacterium radiobacter
Southern blotting
lowlands
phosphotransferases (kinases)
hybridization
inflorescences
fruits
cultivars

Keywords

  • Agrobacterium
  • Biofuel crop
  • Panicum virgatum
  • Switchgrass
  • Transgene inheritance
  • Transgenic plant

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Energy (miscellaneous)
  • Renewable Energy, Sustainability and the Environment

Cite this

Agrobacterium-mediated transformation of switchgrass and inheritance of the transgenes. / Xi, Yajun; Fu, Chunxiang; Ge, Yaxin; Nandakumar, Rangaraj; Hisano, Hiroshi; Bouton, Joseph; Wang, Zeng Yu.

In: Bioenergy Research, Vol. 2, No. 4, 11.2009, p. 275-283.

Research output: Contribution to journalArticle

Xi, Yajun ; Fu, Chunxiang ; Ge, Yaxin ; Nandakumar, Rangaraj ; Hisano, Hiroshi ; Bouton, Joseph ; Wang, Zeng Yu. / Agrobacterium-mediated transformation of switchgrass and inheritance of the transgenes. In: Bioenergy Research. 2009 ; Vol. 2, No. 4. pp. 275-283.
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