Aging effects on passive resistive torque in the rat ankle joint after lengthening contractions

Eisuke Ochi, Koichi Nakazato, Hongsun Song, Hiroyuki Nakajima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background. The aim of this study was to evaluate whether aging affects (1) the sensation of joint stiffness after lengthening contractions (LCs); (2) passive resistive torque (PRT) of the ankle joint; (3) and the connectin (titin) isoform composition in the medial gastrocnemius (MG) muscle. Methods. Anesthetized young (9 weeks) and adult (35 weeks) Wistar rats (n = 6 per group) were used in the present study. A single bout of LCs was performed on the MG muscle with a combination of electrically induced tetanic contractions via a skin electrode and simultaneous forced dorsiflexion of the ankle joint (angular velocity 15°/s, 0°-45°). Static PRT of the ankle joint (at 30° and 45°) was measured to evaluate joint stiffness. These parameters were measured until 10 days after treatment. The connectin isoform was measured as muscle extensibility employing low-porosity 2% polyacrylamide gel electrophoresis. Results. The averaged static PRT was significantly higher in adult rats than in young rats. Connectin isoform analysis revealed that the adult group contained larger amounts of β-connectin than did the young rats. After the LCs, static PRT of the ankle joint gradually increased until day 4 in the young group, whereas the adult group did not show a significant change during the experimental period. Conclusions. We concluded that (1) joint stiffness of adult rats is greater than in young rats, similar to human studies; (2) the increased joint stiffness may be due to connectin isoform composition; and (3) joint stiffness after MG muscle LCs is more apparent in young rats.

Original languageEnglish
Pages (from-to)218-224
Number of pages7
JournalJournal of Orthopaedic Science
Volume13
Issue number3
DOIs
Publication statusPublished - May 2008
Externally publishedYes

Fingerprint

Connectin
Ankle Joint
Torque
Joints
Protein Isoforms
Skeletal Muscle
Porosity
Muscle Contraction
Wistar Rats
Polyacrylamide Gel Electrophoresis
Electrodes
Muscles
Skin

ASJC Scopus subject areas

  • Surgery

Cite this

Aging effects on passive resistive torque in the rat ankle joint after lengthening contractions. / Ochi, Eisuke; Nakazato, Koichi; Song, Hongsun; Nakajima, Hiroyuki.

In: Journal of Orthopaedic Science, Vol. 13, No. 3, 05.2008, p. 218-224.

Research output: Contribution to journalArticle

Ochi, Eisuke ; Nakazato, Koichi ; Song, Hongsun ; Nakajima, Hiroyuki. / Aging effects on passive resistive torque in the rat ankle joint after lengthening contractions. In: Journal of Orthopaedic Science. 2008 ; Vol. 13, No. 3. pp. 218-224.
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