Aggregation ability of virus-specific antibodies is correlated with their capacity to neutralize rice dwarf virus

Hongyan Chen, Taiyun Wei, Ai Saotome, Kengo Sakaguchi, Yasuo Shikamoto, Hiroshi Mizuno, Fusamichi Akita, Takumi Shimizu, Tamaki Uehara-Ichiki, Toshihiro Omura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Antibodies (immunoglobulin G (IgGs)) from antisera raised against viral particles that had been dissociated by treatment with SDS and intact particles of Rice dwarf virus (RDV) were studied for their ability to prevent viral infection of vector cells in monolayers in vitro. Even though IgGs raised against dissociated virus had a higher titer than those raised against intact viruses in an analysis of viral proteins on Western blots, they did not neutralize RDV. Conversely, IgGs raised against intact RDV effectively neutralized viral infectivity. Electron microscopic observation of the aggregation of RDV particles after incubation with IgGs raised against intact RDV, but no aggregation of RDV particles after incubation with IgGs raised against dissociated RDV suggested that IgGs raised against intact viruses might prevent viral invasion by causing clumping of viruses, thereby reducing the number of infectious units. Our results reveal, for the first time, a possible mechanism for the neutralization, mediated by antibodies, of plant viruses that propagate in insect vector cells.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalJapan Agricultural Research Quarterly
Volume46
Issue number1
DOIs
Publication statusPublished - Jan 2012
Externally publishedYes

Fingerprint

Rice dwarf virus
immunoglobulin G
antibody
virus
rice
Viruses
viruses
antibodies
Antibodies
Immunoglobulin G
virion
Virion
virus particle
insect vectors
viral proteins
plant viruses
Insect Vectors
incubation
Oryza
Plant Viruses

Keywords

  • Vector cell monolayer
  • Viral aggregation
  • Viral detection

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Animal Science and Zoology
  • Ecology
  • Biotechnology

Cite this

Aggregation ability of virus-specific antibodies is correlated with their capacity to neutralize rice dwarf virus. / Chen, Hongyan; Wei, Taiyun; Saotome, Ai; Sakaguchi, Kengo; Shikamoto, Yasuo; Mizuno, Hiroshi; Akita, Fusamichi; Shimizu, Takumi; Uehara-Ichiki, Tamaki; Omura, Toshihiro.

In: Japan Agricultural Research Quarterly, Vol. 46, No. 1, 01.2012, p. 65-71.

Research output: Contribution to journalArticle

Chen, H, Wei, T, Saotome, A, Sakaguchi, K, Shikamoto, Y, Mizuno, H, Akita, F, Shimizu, T, Uehara-Ichiki, T & Omura, T 2012, 'Aggregation ability of virus-specific antibodies is correlated with their capacity to neutralize rice dwarf virus', Japan Agricultural Research Quarterly, vol. 46, no. 1, pp. 65-71. https://doi.org/10.6090/jarq.46.65
Chen, Hongyan ; Wei, Taiyun ; Saotome, Ai ; Sakaguchi, Kengo ; Shikamoto, Yasuo ; Mizuno, Hiroshi ; Akita, Fusamichi ; Shimizu, Takumi ; Uehara-Ichiki, Tamaki ; Omura, Toshihiro. / Aggregation ability of virus-specific antibodies is correlated with their capacity to neutralize rice dwarf virus. In: Japan Agricultural Research Quarterly. 2012 ; Vol. 46, No. 1. pp. 65-71.
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