Aggregatibacter actinomycetemcomitans regulates the expression of integrins and reduces cell adhesion via integrin α5 in human gingival epithelial cells

Shinsuke Kochi, Keisuke Yamashiro, Shoichi Hongo, Tadashi Yamamoto, Yuki Ugawa, Masayuki Shimoe, Mari Kawamura, Chiaki Hirata-Yoshihara, Hidetaka Ideguchi, Hiroshi Maeda, Shogo Takashiba

Research output: Contribution to journalArticle

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Abstract

Gingival epithelial cells form a physiological barrier against bacterial invasion. Excessive bacterial invasion destroys the attachment between the tooth surface and the epithelium, resulting in periodontitis. Integrins play a significant role in cell attachment; therefore, we hypothesized that bacterial infection might decrease the expressions of these integrins in gingival epithelial cells, resulting in reduced cell adhesion. Immortalized human gingival epithelial cells were co-cultured with Aggregatibacter actinomycetemcomitans Y4 (Aa Y4), and the gene expression levels of IL-8, proliferating cell nuclear antigen (PCNA), and integrins (α2, α3, α5, β4, and β6) were measured using quantitative reverse transcription polymerase chain reaction. Expression of PCNA and integrins, except integrin α5, was significantly downregulated, while expression of IL-8 and integrin α5 was significantly upregulated in the cells co-cultured with Aa Y4. The number of adherent cells significantly decreased when co-cultured with Aa Y4, as determined using cell adhesion assays. In the cells co-cultured with Aa Y4 and an integrin α5 neutralizing antibody, there was no effect on the expression of IL-8 and PCNA, while the expressions of integrins α2, α3, β4, and β6, and the number of adherent cells did not decrease. The number of invading bacteria in the cells was reduced in the presence of the antibody and increased in the presence of TLR2/4 inhibitor. Therefore, integrin α5 might be involved in Aa Y4 invasion into gingival epithelial cells, and the resulting signal transduction cascade reduces cell adhesion by decreasing the expression of integrins, while the TLR2/4 signaling cascade regulates IL-8 expression.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalMolecular and Cellular Biochemistry
DOIs
Publication statusAccepted/In press - Jun 7 2017

Fingerprint

Aggregatibacter actinomycetemcomitans
Cell adhesion
Cell Adhesion
Integrins
Epithelial Cells
Interleukin-8
Proliferating Cell Nuclear Antigen
Cultured Cells
Cell Count
Signal transduction
Periodontitis
Polymerase chain reaction
Transcription
Neutralizing Antibodies
Bacterial Infections
Gene expression
Reverse Transcription
Assays
Signal Transduction
Bacteria

Keywords

  • Aggregatibacter actinomycetemcomitans
  • Cell adhesion
  • Gingival epithelial cell
  • Infection model
  • Integrin
  • Periodontal disease

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Aggregatibacter actinomycetemcomitans regulates the expression of integrins and reduces cell adhesion via integrin α5 in human gingival epithelial cells. / Kochi, Shinsuke; Yamashiro, Keisuke; Hongo, Shoichi; Yamamoto, Tadashi; Ugawa, Yuki; Shimoe, Masayuki; Kawamura, Mari; Hirata-Yoshihara, Chiaki; Ideguchi, Hidetaka; Maeda, Hiroshi; Takashiba, Shogo.

In: Molecular and Cellular Biochemistry, 07.06.2017, p. 1-10.

Research output: Contribution to journalArticle

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AU - Hongo, Shoichi

AU - Yamamoto, Tadashi

AU - Ugawa, Yuki

AU - Shimoe, Masayuki

AU - Kawamura, Mari

AU - Hirata-Yoshihara, Chiaki

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AU - Maeda, Hiroshi

AU - Takashiba, Shogo

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