Aggregated chromosomes transfer in human oocytes

Junko Otsuki; Yasushi Nagai; Tadashi Sankai

doi:10.1016/j.rbmo.2013.10.024

Aggregated chromosomes transfer in human oocytes

Junko Otsuki, Yasushi Nagai, Tadashi Sankai

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Germinal-vesicle (GV) transfer, spindle-chromosome complex transfer in metaphase-II oocytes and two pronuclei transfer have been evaluated as possible treatments for patients who have mitochondrial diseases. However, GV transfers often lead to heteroplasmy while the other two methods are frequently associated with aneuploidy. The present study used a new method based on the transfer of aggregated chromosomes, which occurs in human oocytes, before the metaphase spindle is established.

Original language	English
Pages (from-to)	401-404
Number of pages	4
Journal	Reproductive BioMedicine Online
Volume	28
Issue number	3
DOIs	https://doi.org/10.1016/j.rbmo.2013.10.024
Publication status	Published - Mar 2014
Externally published	Yes

Keywords

aggregated chromosomes
aneuploidy
heteroplasmy
microinjection
mitochondrial disease
nuclear transfer

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynaecology
Developmental Biology

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AU - Otsuki, Junko

AU - Nagai, Yasushi

AU - Sankai, Tadashi

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AB - Germinal-vesicle (GV) transfer, spindle-chromosome complex transfer in metaphase-II oocytes and two pronuclei transfer have been evaluated as possible treatments for patients who have mitochondrial diseases. However, GV transfers often lead to heteroplasmy while the other two methods are frequently associated with aneuploidy. The present study used a new method based on the transfer of aggregated chromosomes, which occurs in human oocytes, before the metaphase spindle is established.

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KW - aneuploidy

KW - heteroplasmy

KW - microinjection

KW - mitochondrial disease

KW - nuclear transfer

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