Abstract
Germinal-vesicle (GV) transfer, spindle-chromosome complex transfer in metaphase-II oocytes and two pronuclei transfer have been evaluated as possible treatments for patients who have mitochondrial diseases. However, GV transfers often lead to heteroplasmy while the other two methods are frequently associated with aneuploidy. The present study used a new method based on the transfer of aggregated chromosomes, which occurs in human oocytes, before the metaphase spindle is established.
| Original language | English |
|---|---|
| Pages (from-to) | 401-404 |
| Number of pages | 4 |
| Journal | Reproductive BioMedicine Online |
| Volume | 28 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2014 |
| Externally published | Yes |
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Keywords
- aggregated chromosomes
- aneuploidy
- heteroplasmy
- microinjection
- mitochondrial disease
- nuclear transfer
ASJC Scopus subject areas
- Reproductive Medicine
- Developmental Biology
Cite this
Aggregated chromosomes transfer in human oocytes. / Otsuki, Junko; Nagai, Yasushi; Sankai, Tadashi.
In: Reproductive BioMedicine Online, Vol. 28, No. 3, 2014, p. 401-404.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aggregated chromosomes transfer in human oocytes
AU - Otsuki, Junko
AU - Nagai, Yasushi
AU - Sankai, Tadashi
PY - 2014
Y1 - 2014
N2 - Germinal-vesicle (GV) transfer, spindle-chromosome complex transfer in metaphase-II oocytes and two pronuclei transfer have been evaluated as possible treatments for patients who have mitochondrial diseases. However, GV transfers often lead to heteroplasmy while the other two methods are frequently associated with aneuploidy. The present study used a new method based on the transfer of aggregated chromosomes, which occurs in human oocytes, before the metaphase spindle is established.
AB - Germinal-vesicle (GV) transfer, spindle-chromosome complex transfer in metaphase-II oocytes and two pronuclei transfer have been evaluated as possible treatments for patients who have mitochondrial diseases. However, GV transfers often lead to heteroplasmy while the other two methods are frequently associated with aneuploidy. The present study used a new method based on the transfer of aggregated chromosomes, which occurs in human oocytes, before the metaphase spindle is established.
KW - aggregated chromosomes
KW - aneuploidy
KW - heteroplasmy
KW - microinjection
KW - mitochondrial disease
KW - nuclear transfer
UR - http://www.scopus.com/inward/record.url?scp=84895467318&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84895467318&partnerID=8YFLogxK
U2 - 10.1016/j.rbmo.2013.10.024
DO - 10.1016/j.rbmo.2013.10.024
M3 - Article
C2 - 24316045
AN - SCOPUS:84895467318
VL - 28
SP - 401
EP - 404
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
SN - 1472-6483
IS - 3
ER -