Abstract
Objectives: Streptococcus mutans can aggravate colitis in mice. We evaluated the virulence of colitis using type strains as well as blood isolates of several oral streptococcal species. Materials and Methods: We investigated the susceptibility of blood isolates of several oral streptococci to phagocytosis, adhesion to and invasion of hepatic cells and interferon-γ secretion. A mouse model of dextran sodium sulphate-induced colitis was used to evaluate bacterial aggravation of colitis. In addition, interferon-γ antibody was administered to mice with prominent aggravation of colitis. Results: In vitro analyses showed that Streptococcus sanguinis ATCC 10556 was a possible virulent strain among type strains of several oral streptococci, and that analysis of blood isolates of S. sanguinis TW289 revealed a potential virulent strain. Intravenous administration of ATCC 10556 and TW289 caused prominent aggravation of dextran sodium sulphate-induced colitis, and histopathological examinations showed that interferon-γ secretion due to infection of hepatic cells caused colitis aggravation. Administration of interferon-γ antibody suppressed TW289-induced colitis. Conclusion: These results suggest that some virulent oral streptococcal strains are associated with the aggravation of colitis induced by enhanced secretion of interferon-γ when they invade the bloodstream.
| Original language | English |
|---|---|
| Pages (from-to) | 359-366 |
| Number of pages | 8 |
| Journal | Oral Diseases |
| Volume | 20 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2014 |
| Externally published | Yes |
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Keywords
- Bacteraemia
- Inflammatory bowel diseases
- Interferon-gamma
- Liver
- Oral streptococci
ASJC Scopus subject areas
- Dentistry(all)
- Otorhinolaryngology
- Medicine(all)
Cite this
Aggravation of inflammatory bowel diseases by oral streptococci. / Kojima, A.; Nomura, R.; Naka, Shuhei; Okawa, R.; Ooshima, T.; Nakano, K.
In: Oral Diseases, Vol. 20, No. 4, 2014, p. 359-366.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aggravation of inflammatory bowel diseases by oral streptococci
AU - Kojima, A.
AU - Nomura, R.
AU - Naka, Shuhei
AU - Okawa, R.
AU - Ooshima, T.
AU - Nakano, K.
PY - 2014
Y1 - 2014
N2 - Objectives: Streptococcus mutans can aggravate colitis in mice. We evaluated the virulence of colitis using type strains as well as blood isolates of several oral streptococcal species. Materials and Methods: We investigated the susceptibility of blood isolates of several oral streptococci to phagocytosis, adhesion to and invasion of hepatic cells and interferon-γ secretion. A mouse model of dextran sodium sulphate-induced colitis was used to evaluate bacterial aggravation of colitis. In addition, interferon-γ antibody was administered to mice with prominent aggravation of colitis. Results: In vitro analyses showed that Streptococcus sanguinis ATCC 10556 was a possible virulent strain among type strains of several oral streptococci, and that analysis of blood isolates of S. sanguinis TW289 revealed a potential virulent strain. Intravenous administration of ATCC 10556 and TW289 caused prominent aggravation of dextran sodium sulphate-induced colitis, and histopathological examinations showed that interferon-γ secretion due to infection of hepatic cells caused colitis aggravation. Administration of interferon-γ antibody suppressed TW289-induced colitis. Conclusion: These results suggest that some virulent oral streptococcal strains are associated with the aggravation of colitis induced by enhanced secretion of interferon-γ when they invade the bloodstream.
AB - Objectives: Streptococcus mutans can aggravate colitis in mice. We evaluated the virulence of colitis using type strains as well as blood isolates of several oral streptococcal species. Materials and Methods: We investigated the susceptibility of blood isolates of several oral streptococci to phagocytosis, adhesion to and invasion of hepatic cells and interferon-γ secretion. A mouse model of dextran sodium sulphate-induced colitis was used to evaluate bacterial aggravation of colitis. In addition, interferon-γ antibody was administered to mice with prominent aggravation of colitis. Results: In vitro analyses showed that Streptococcus sanguinis ATCC 10556 was a possible virulent strain among type strains of several oral streptococci, and that analysis of blood isolates of S. sanguinis TW289 revealed a potential virulent strain. Intravenous administration of ATCC 10556 and TW289 caused prominent aggravation of dextran sodium sulphate-induced colitis, and histopathological examinations showed that interferon-γ secretion due to infection of hepatic cells caused colitis aggravation. Administration of interferon-γ antibody suppressed TW289-induced colitis. Conclusion: These results suggest that some virulent oral streptococcal strains are associated with the aggravation of colitis induced by enhanced secretion of interferon-γ when they invade the bloodstream.
KW - Bacteraemia
KW - Inflammatory bowel diseases
KW - Interferon-gamma
KW - Liver
KW - Oral streptococci
UR - http://www.scopus.com/inward/record.url?scp=84897530070&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897530070&partnerID=8YFLogxK
U2 - 10.1111/odi.12125
DO - 10.1111/odi.12125
M3 - Article
C2 - 23679203
AN - SCOPUS:84897530070
VL - 20
SP - 359
EP - 366
JO - Oral Diseases
JF - Oral Diseases
SN - 1354-523X
IS - 4
ER -