Age-related methylation in normal colon mucosa differs between the proximal and distal colon in patients who underwent colonoscopy

Joichiro Horii, Sakiko Hiraoka, Jun Kato, Keita Harada, Kenji Kuwaki, Hideyuki Fujita, Shinichi Toyooka, Kazuhide Yamamoto

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objectives: To examine the difference in the methylation status in normal colon mucosa between the proximal and distal colon, in relation to the correlation between the methylation status of normal mucosa and characteristics of neoplasia. Design and methods: Paired biopsy specimens of normal mucosa from the proximal and distal colon of 82 patients who underwent colonoscopy were obtained. The methylation status of the promoter region of estrogen receptor 1 (ESR1) and myogenic differentiation 1 (MYOD1) was examined. Results: Normal mucosa was more highly methylated in the distal than in the proximal colon in both ESR1 and MYOD1 loci (p < 0.0001 and p = 0.0009, respectively). Advanced characteristics of polyps in the distal colon were frequently observed in patients with lower methylation of ESR1 in the distal colon normal mucosa. Conclusions: Methylation levels in normal mucosa differ between the proximal and distal colon, and lower methylation of ESR1 in the distal colon normal mucosa may correlate with advanced features of neoplasia in the distal colon.

Original languageEnglish
Pages (from-to)1440-1448
Number of pages9
JournalClinical Biochemistry
Volume41
Issue number18
DOIs
Publication statusPublished - Dec 2008

Keywords

  • Colon
  • ESR1
  • MethyLight
  • Methylation

ASJC Scopus subject areas

  • Clinical Biochemistry

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