Age-related changes in expression of metallothionein-III in rat brain

Ikuko Miyazaki, Masato Asanuma, Youichirou Higashi, Chiharu Sogawa, Ken Ichi Tanaka, Norio Ogawa

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Metallothionein (MT)-III is a metal binding protein, called growth inhibitory factor, and is mainly expressed in the central nervous system. Since MT-III decreases in the brain of Alzheimer's disease (AD), a growing interest has been focused on its relationship to neurodegenerative diseases. To clarify age-related changes in the MT-III expression and its inducibility against oxidative stress, we analyzed the expression of MT-III and its mRNA in the brain of lipopolysaccharide (LPS)-treated aged rats. In the frontal cortex, basal expression of MT-III mRNA was significantly increased with aging, while it was observed no induction of MT-III mRNA against LPS administration in the aged rat brain. MT-III immunopositive cells were increased in the frontal, parietal and piriform cortices, hypothalamus and amygdaloid nucleus with aging. The LPS treatment induced MT-III expression in the brain of young-adult rats, but not in the aged rat brain. Furthermore, the MT-III induction with LPS treatment was mainly observed in oligodendrocyte and microglia. In the present study, we showed that inducibility of brain MT-III against oxidative stress may be reduced with aging. Since it has been reported that MT-III has neuroprotective roles as an antioxidant, present results suggest that MT-III is closely related to the neurodegeneration in the aged animals.

Original languageEnglish
Pages (from-to)323-333
Number of pages11
JournalNeuroscience Research
Volume43
Issue number4
DOIs
Publication statusPublished - 2002

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Brain
Lipopolysaccharides
Frontal Lobe
growth inhibitory factor
Messenger RNA
Oxidative Stress
Parietal Lobe
Oligodendroglia
Microglia
Brain Diseases
Amygdala
Neurodegenerative Diseases
Hypothalamus
Young Adult
Carrier Proteins
Alzheimer Disease
Central Nervous System
Antioxidants
Metals
Therapeutics

Keywords

  • Growth inhibitory factor (GIF)
  • Metallothionein-III
  • Oxidative stress

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Age-related changes in expression of metallothionein-III in rat brain. / Miyazaki, Ikuko; Asanuma, Masato; Higashi, Youichirou; Sogawa, Chiharu; Tanaka, Ken Ichi; Ogawa, Norio.

In: Neuroscience Research, Vol. 43, No. 4, 2002, p. 323-333.

Research output: Contribution to journalArticle

Miyazaki, Ikuko ; Asanuma, Masato ; Higashi, Youichirou ; Sogawa, Chiharu ; Tanaka, Ken Ichi ; Ogawa, Norio. / Age-related changes in expression of metallothionein-III in rat brain. In: Neuroscience Research. 2002 ; Vol. 43, No. 4. pp. 323-333.
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