Age-related changes in composition of transcription factor, AP-1 complex in the rat brain

Masato Asanuma, Yoichi Kondo, Sakiko Nishibayashi, Emi Iwata, Tohru Nakanishi, Norio Ogawa

Research output: Contribution to journalArticle

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Abstract

We examined age-related changes in composition of transcription factor, activator protein-1 (AP-1) which binds to TPA responsive element (TRE) in the non-stimulated rat brain, using electrophoretic mobility-shift assay with immunodepletion/supershift assay. The total TRE-binding activity in the frontal cortex and the hippocampus of the aged rats markedly decreased to 66% and 43%, respectively, and TRE-bindings of AP-1 in both regions also decreased to 82% and 66%, respectively, with aging. Jun-Jun dimers accounted for approximately half of the total TRE-bindings and 80-90% of the AP-1 bindings, while there were fewer Fos-Jun dimers, in both examined regions of the non-stimulated adult. The proportion of active Fos-Jun heterodimers in the frontal cortex increased to up to half of the AP-1 bindings in the aged rats, indicating that cortical AP-1-related transcription may increase with aging even under the non-stimulated condition. In the hippocampus, inactive Jun-Jun homodimers became predominant in AP-1 with aging. This regional diversity of age-related changes in the composition of AP-1 in the brain may be related to changes or dysfunction in neuronal signal transduction in the aged.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalNeuroscience Letters
Volume201
Issue number2
DOIs
Publication statusPublished - Dec 8 1995

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Keywords

  • Activator protein-1 (AP-1)
  • Aging
  • Composition of AP-1
  • Electrophoretic mobility-shift assay
  • Fos-June heterodimer
  • Jun-Jun homodimer
  • Rat brain

ASJC Scopus subject areas

  • Neuroscience(all)

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