TY - JOUR
T1 - Age-related changes in composition of transcription factor, AP-1 complex in the rat brain
AU - Asanuma, Masato
AU - Kondo, Yoichi
AU - Nishibayashi, Sakiko
AU - Iwata, Emi
AU - Nakanishi, Tohru
AU - Ogawa, Norio
PY - 1995/12/8
Y1 - 1995/12/8
N2 - We examined age-related changes in composition of transcription factor, activator protein-1 (AP-1) which binds to TPA responsive element (TRE) in the non-stimulated rat brain, using electrophoretic mobility-shift assay with immunodepletion/supershift assay. The total TRE-binding activity in the frontal cortex and the hippocampus of the aged rats markedly decreased to 66% and 43%, respectively, and TRE-bindings of AP-1 in both regions also decreased to 82% and 66%, respectively, with aging. Jun-Jun dimers accounted for approximately half of the total TRE-bindings and 80-90% of the AP-1 bindings, while there were fewer Fos-Jun dimers, in both examined regions of the non-stimulated adult. The proportion of active Fos-Jun heterodimers in the frontal cortex increased to up to half of the AP-1 bindings in the aged rats, indicating that cortical AP-1-related transcription may increase with aging even under the non-stimulated condition. In the hippocampus, inactive Jun-Jun homodimers became predominant in AP-1 with aging. This regional diversity of age-related changes in the composition of AP-1 in the brain may be related to changes or dysfunction in neuronal signal transduction in the aged.
AB - We examined age-related changes in composition of transcription factor, activator protein-1 (AP-1) which binds to TPA responsive element (TRE) in the non-stimulated rat brain, using electrophoretic mobility-shift assay with immunodepletion/supershift assay. The total TRE-binding activity in the frontal cortex and the hippocampus of the aged rats markedly decreased to 66% and 43%, respectively, and TRE-bindings of AP-1 in both regions also decreased to 82% and 66%, respectively, with aging. Jun-Jun dimers accounted for approximately half of the total TRE-bindings and 80-90% of the AP-1 bindings, while there were fewer Fos-Jun dimers, in both examined regions of the non-stimulated adult. The proportion of active Fos-Jun heterodimers in the frontal cortex increased to up to half of the AP-1 bindings in the aged rats, indicating that cortical AP-1-related transcription may increase with aging even under the non-stimulated condition. In the hippocampus, inactive Jun-Jun homodimers became predominant in AP-1 with aging. This regional diversity of age-related changes in the composition of AP-1 in the brain may be related to changes or dysfunction in neuronal signal transduction in the aged.
KW - Activator protein-1 (AP-1)
KW - Aging
KW - Composition of AP-1
KW - Electrophoretic mobility-shift assay
KW - Fos-June heterodimer
KW - Jun-Jun homodimer
KW - Rat brain
UR - http://www.scopus.com/inward/record.url?scp=0028811056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028811056&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(95)12152-8
DO - 10.1016/0304-3940(95)12152-8
M3 - Article
C2 - 8848234
AN - SCOPUS:0028811056
VL - 201
SP - 127
EP - 130
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -