Age effects on ectopic bone formation induced by purified bone morphogenetic protein

Noriyuki Nagai, Chun Lin Qin, Hitoshi Nagatsuka, Masahisa Inoue, Yuzo Ishiwari

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A mixture of heparin-Sepharose-purified bovine bone morphogenetic protein and type I atellocollagen was implanted in the subcutaneous tissues of 4-week, 10-month and 18-month-old rats. The implants were removed at 7, 14 and 21 days after implantation. The effects of rat age on ectopic bone formation were evaluated on the explants using haematoxylin-eosin staining, morphometric analysis, alkaline phosphatase activity and calcium content determination, as well as immunohistochemical staining of type IV collagen present in the basement membrane of blood vessels. On day 14 and 21, bone was observed in 4-week and 10-month-old rats, but the amount of bone formed in the latter was less than in the 4-week-old rats. In 18-month-old rats, bone was first found focally in very limited regions of the explants on day 21 and the amount of bone was much less than in 4-week-old rats. At all periods, akaline phosphatase activity was higher in younger rats. On day 7, there were more blood vessels in the explants of 4-week-old rats than in those of 10-month or 18-month-old rats. On day 14 and 21, more blood vessels were found in the central regions of the explants in 4-week-old rats than in the same regions in 10-month or 18-month-old rats. The findings in the present study indicate that the rate and quantity of ectopic bone formation are reduced in aged rats, and suggest that the difference in blood vessel distribution is related to this reduction in ectopic bone formation.

Original languageEnglish
Pages (from-to)143-150
Number of pages8
JournalInternational Journal of Oral and Maxillofacial Surgery
Volume28
Issue number2
Publication statusPublished - Apr 1999

Fingerprint

Bone Morphogenetic Proteins
Osteogenesis
Blood Vessels
Bone and Bones
Staining and Labeling
Collagen Type IV
Subcutaneous Tissue
Hematoxylin
Eosine Yellowish-(YS)
Phosphoric Monoester Hydrolases
Basement Membrane
Alkaline Phosphatase

Keywords

  • Aging
  • Blood vessel
  • Bone morphogenetic protein
  • Ectopic bone
  • Immunohistochemistry

ASJC Scopus subject areas

  • Dentistry(all)
  • Otorhinolaryngology
  • Surgery

Cite this

Age effects on ectopic bone formation induced by purified bone morphogenetic protein. / Nagai, Noriyuki; Qin, Chun Lin; Nagatsuka, Hitoshi; Inoue, Masahisa; Ishiwari, Yuzo.

In: International Journal of Oral and Maxillofacial Surgery, Vol. 28, No. 2, 04.1999, p. 143-150.

Research output: Contribution to journalArticle

Nagai, Noriyuki ; Qin, Chun Lin ; Nagatsuka, Hitoshi ; Inoue, Masahisa ; Ishiwari, Yuzo. / Age effects on ectopic bone formation induced by purified bone morphogenetic protein. In: International Journal of Oral and Maxillofacial Surgery. 1999 ; Vol. 28, No. 2. pp. 143-150.
@article{cab9de3517bc4afd8ba7277dfffa0b55,
title = "Age effects on ectopic bone formation induced by purified bone morphogenetic protein",
abstract = "A mixture of heparin-Sepharose-purified bovine bone morphogenetic protein and type I atellocollagen was implanted in the subcutaneous tissues of 4-week, 10-month and 18-month-old rats. The implants were removed at 7, 14 and 21 days after implantation. The effects of rat age on ectopic bone formation were evaluated on the explants using haematoxylin-eosin staining, morphometric analysis, alkaline phosphatase activity and calcium content determination, as well as immunohistochemical staining of type IV collagen present in the basement membrane of blood vessels. On day 14 and 21, bone was observed in 4-week and 10-month-old rats, but the amount of bone formed in the latter was less than in the 4-week-old rats. In 18-month-old rats, bone was first found focally in very limited regions of the explants on day 21 and the amount of bone was much less than in 4-week-old rats. At all periods, akaline phosphatase activity was higher in younger rats. On day 7, there were more blood vessels in the explants of 4-week-old rats than in those of 10-month or 18-month-old rats. On day 14 and 21, more blood vessels were found in the central regions of the explants in 4-week-old rats than in the same regions in 10-month or 18-month-old rats. The findings in the present study indicate that the rate and quantity of ectopic bone formation are reduced in aged rats, and suggest that the difference in blood vessel distribution is related to this reduction in ectopic bone formation.",
keywords = "Aging, Blood vessel, Bone morphogenetic protein, Ectopic bone, Immunohistochemistry",
author = "Noriyuki Nagai and Qin, {Chun Lin} and Hitoshi Nagatsuka and Masahisa Inoue and Yuzo Ishiwari",
year = "1999",
month = "4",
language = "English",
volume = "28",
pages = "143--150",
journal = "International Journal of Oral and Maxillofacial Surgery",
issn = "0901-5027",
publisher = "Churchill Livingstone",
number = "2",

}

TY - JOUR

T1 - Age effects on ectopic bone formation induced by purified bone morphogenetic protein

AU - Nagai, Noriyuki

AU - Qin, Chun Lin

AU - Nagatsuka, Hitoshi

AU - Inoue, Masahisa

AU - Ishiwari, Yuzo

PY - 1999/4

Y1 - 1999/4

N2 - A mixture of heparin-Sepharose-purified bovine bone morphogenetic protein and type I atellocollagen was implanted in the subcutaneous tissues of 4-week, 10-month and 18-month-old rats. The implants were removed at 7, 14 and 21 days after implantation. The effects of rat age on ectopic bone formation were evaluated on the explants using haematoxylin-eosin staining, morphometric analysis, alkaline phosphatase activity and calcium content determination, as well as immunohistochemical staining of type IV collagen present in the basement membrane of blood vessels. On day 14 and 21, bone was observed in 4-week and 10-month-old rats, but the amount of bone formed in the latter was less than in the 4-week-old rats. In 18-month-old rats, bone was first found focally in very limited regions of the explants on day 21 and the amount of bone was much less than in 4-week-old rats. At all periods, akaline phosphatase activity was higher in younger rats. On day 7, there were more blood vessels in the explants of 4-week-old rats than in those of 10-month or 18-month-old rats. On day 14 and 21, more blood vessels were found in the central regions of the explants in 4-week-old rats than in the same regions in 10-month or 18-month-old rats. The findings in the present study indicate that the rate and quantity of ectopic bone formation are reduced in aged rats, and suggest that the difference in blood vessel distribution is related to this reduction in ectopic bone formation.

AB - A mixture of heparin-Sepharose-purified bovine bone morphogenetic protein and type I atellocollagen was implanted in the subcutaneous tissues of 4-week, 10-month and 18-month-old rats. The implants were removed at 7, 14 and 21 days after implantation. The effects of rat age on ectopic bone formation were evaluated on the explants using haematoxylin-eosin staining, morphometric analysis, alkaline phosphatase activity and calcium content determination, as well as immunohistochemical staining of type IV collagen present in the basement membrane of blood vessels. On day 14 and 21, bone was observed in 4-week and 10-month-old rats, but the amount of bone formed in the latter was less than in the 4-week-old rats. In 18-month-old rats, bone was first found focally in very limited regions of the explants on day 21 and the amount of bone was much less than in 4-week-old rats. At all periods, akaline phosphatase activity was higher in younger rats. On day 7, there were more blood vessels in the explants of 4-week-old rats than in those of 10-month or 18-month-old rats. On day 14 and 21, more blood vessels were found in the central regions of the explants in 4-week-old rats than in the same regions in 10-month or 18-month-old rats. The findings in the present study indicate that the rate and quantity of ectopic bone formation are reduced in aged rats, and suggest that the difference in blood vessel distribution is related to this reduction in ectopic bone formation.

KW - Aging

KW - Blood vessel

KW - Bone morphogenetic protein

KW - Ectopic bone

KW - Immunohistochemistry

UR - http://www.scopus.com/inward/record.url?scp=0033115886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033115886&partnerID=8YFLogxK

M3 - Article

VL - 28

SP - 143

EP - 150

JO - International Journal of Oral and Maxillofacial Surgery

JF - International Journal of Oral and Maxillofacial Surgery

SN - 0901-5027

IS - 2

ER -