Age-dependent vulnerability to ischemia-reperfusion injury of cyanotic myocardium in a chronic hypoxic rat model

Yasufumi Fujita, Kozo Ishino, Koji Nakanishi, Yasuhiro Fujii, Masaaki Kawada, Shunji Sano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study evaluated the effects of chronic hypoxia from birth on the resistance of rat hearts to global ischemia, with special emphasis on the duration of hypoxia. Male Wistar rats were housed from birth for 4 weeks or 8 weeks either in a hypoxic environment (FiO2 = 0.12) or in ambient air (8 animals for each group). Isolated rat hearts were perfused for 40 min with oxygenated Krebs-Henseleit buffer, subjected to 20 min global no-flow ischemia at 37°C, and then underwent 40 min of reperfusion. A non-elastic balloon was inserted into the left ventricle and inflated until the pre-ischemic LVEDP rose to 8mmHg. Cardiac function was measured before and after ischemia. The post-ischemic percent recovery of LVDP in hypoxic hearts was worse than in normoxic hearts (4 weeks: 55 ± 7 vs. 96 ± 3%, p<0.01; 8 weeks: 40 ±5 vs. 92 ± 4%, p

Original languageEnglish
Pages (from-to)237-242
Number of pages6
JournalActa Medica Okayama
Volume63
Issue number5
Publication statusPublished - Nov 2009

Fingerprint

Reperfusion Injury
Rats
Myocardium
Ischemia
Parturition
Balloons
Reperfusion
Heart Ventricles
Wistar Rats
Animals
Air
Recovery
Hypoxia

Keywords

  • Aging
  • Chronic hypoxia
  • Ischemia-reperfusion injury

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fujita, Y., Ishino, K., Nakanishi, K., Fujii, Y., Kawada, M., & Sano, S. (2009). Age-dependent vulnerability to ischemia-reperfusion injury of cyanotic myocardium in a chronic hypoxic rat model. Acta Medica Okayama, 63(5), 237-242.

Age-dependent vulnerability to ischemia-reperfusion injury of cyanotic myocardium in a chronic hypoxic rat model. / Fujita, Yasufumi; Ishino, Kozo; Nakanishi, Koji; Fujii, Yasuhiro; Kawada, Masaaki; Sano, Shunji.

In: Acta Medica Okayama, Vol. 63, No. 5, 11.2009, p. 237-242.

Research output: Contribution to journalArticle

Fujita, Y, Ishino, K, Nakanishi, K, Fujii, Y, Kawada, M & Sano, S 2009, 'Age-dependent vulnerability to ischemia-reperfusion injury of cyanotic myocardium in a chronic hypoxic rat model', Acta Medica Okayama, vol. 63, no. 5, pp. 237-242.
Fujita, Yasufumi ; Ishino, Kozo ; Nakanishi, Koji ; Fujii, Yasuhiro ; Kawada, Masaaki ; Sano, Shunji. / Age-dependent vulnerability to ischemia-reperfusion injury of cyanotic myocardium in a chronic hypoxic rat model. In: Acta Medica Okayama. 2009 ; Vol. 63, No. 5. pp. 237-242.
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