Age-Associated deterioration in follicular fluid induces a decline in bovine oocyte quality

Shun Takeo, Koji Kimura, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Maternal age affects the quality of oocytes. The present study examined whether follicular fluid (FF) is a casual factor for age-Associated decline in oocyte quality. First, we measured the concentration of advanced glycation end-products (AGE) in FF derived from young (21-45 months; Young-FF) and aged (≥120 months; Aged-FF) cows and found significantly higher concentrations of AGE in Aged-FF than Young-FF. Second, oocytes were collected from ovaries of young or aged cows and cultured in maturation medium containing 10% FF derived from young or aged cows. Regardless of oocyte origin, Aged-FF accelerated nuclear maturation progression and gap junction closure between oocytes and cumulus cells, increased reactive oxygen species (ROS) content and the rate of abnormal fertilisation of oocytes and decreased blastulation rate compared with Young-FF. Furthermore, supplementation of maturation medium with AGE induced similar age-Associated events in oocytes derived from young cows, in that AGE accelerated the progression of nuclear maturation, increased ROS content in oocytes, increased the rate of abnormal fertilisation and decreased blastulation rate. In conclusion, maternal aging increased the concentration of AGE in FF, and both AGE and Aged-FF accelerated nuclear maturation and reduced the developmental competence of oocytes.

Original languageEnglish
Pages (from-to)759-767
Number of pages9
JournalReproduction, Fertility and Development
Volume29
Issue number4
DOIs
Publication statusPublished - 2017

Fingerprint

Follicular Fluid
follicular fluid
Oocytes
oocytes
deterioration
Advanced Glycosylation End Products
cattle
cows
Fertilization
reactive oxygen species
Reactive Oxygen Species
Cumulus Cells
gap junctions
Age Factors
Gap Junctions
Maternal Age
Mental Competency
advanced glycation end products
Ovary
Mothers

Keywords

  • advanced glycation end-products
  • cow
  • maternal aging.

ASJC Scopus subject areas

  • Biotechnology
  • Reproductive Medicine
  • Molecular Biology
  • Animal Science and Zoology
  • Genetics
  • Endocrinology
  • Developmental Biology

Cite this

Age-Associated deterioration in follicular fluid induces a decline in bovine oocyte quality. / Takeo, Shun; Kimura, Koji; Shirasuna, Koumei; Kuwayama, Takehito; Iwata, Hisataka.

In: Reproduction, Fertility and Development, Vol. 29, No. 4, 2017, p. 759-767.

Research output: Contribution to journalArticle

Takeo, Shun ; Kimura, Koji ; Shirasuna, Koumei ; Kuwayama, Takehito ; Iwata, Hisataka. / Age-Associated deterioration in follicular fluid induces a decline in bovine oocyte quality. In: Reproduction, Fertility and Development. 2017 ; Vol. 29, No. 4. pp. 759-767.
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